MMP-2、TIMP-2在肝癌中的表达及临床意义

Expression of MMP-2,TIMP-2 in hepatic cellular cancer and their clinical significance

目的探讨基质金属蛋白酶-2(MMP-2)及其组织抑制剂(TIMP-2)在人肝癌组织中的表达和生物学特性。方法收集手术切除、术前未行其他治疗且临床病理资料完整的54例肝细胞癌(HCC)患者的石蜡组织标本作研究对象,5例因肝外伤切除的正常肝脏组织作为对照组。对石蜡标本进行连续切片,应用免疫组织化学SP法分别检测HCC组织及正常肝组织中MMP-2、TIMP-2的表达情况。结果MMP-2、TIMP-2的阳性表达定位于癌细胞的细胞浆中,呈棕黄色细颗粒状。HCC组织中MMP-2的阳性表达率为75.9%,TIMP-2的阳性表达率为38.9%,MMP-2的阳性表达率高于TIMP-2(P<0.05)。MMP-2表达与病理分期、病理分级和有无门静脉癌栓有关;TIMP-2表达与病理分期、病理分级有关,与有无门静脉癌栓无关。在包膜完整、包膜突破及无包膜组,HCC组织MMP-2的阳性表达率略呈递降,但差异无统计学意义(P>0.05);而TIMP-2的表达率呈递降趋势,且差异有统计学意义(P<0.05)。正常组织中MMP-2和TIMP-2未见阳性表达。结论MMP-2与TIMP-2的表达状态对判断HCC的侵袭转移有重要价值。

Objective To explore the expression and bionomics of matrix metalloproteinase type 2 （MMP-2） and tissue inhibitor of matrix metalloproteinase type 2 （ TIMP-2 ） in hepato cellular carcinoma（ HCC ）. Methods Fifty-four paraffin samples of HCC patients undergone operation but accepted no other therapy before operation with complete clinical data were collected in this study. Five paraffin samples of normal liver tissue obtained from surgical trauma patients were collected as control group. The expression of MMP-2 and TIMP-2 in HCC and normal hepatic tissue was detected by SP immunohistechemistry. Results The buffy granulose granulase objects were judged as positive stainings ,and the positive expression of MMP-2 and TIMP-2 was found in cytoplasm of HCC ceils. In HCC, the positive percent of TIMP-2 was 38.9% , and MMP- 2 was 75.9% which was higher than that of TIMP-2 （ P 〈 0. 05 ）. The correlation was found between the expression of MMP-2 and pathological stage,pathological grade and portal vein cancer embolus . The expression of TIMP-2 had a relationship with pathological stage and pathological grade, but no relationship with portal vein cancer embolus. The positive expression of MMP-2 and TIMP-2 declined in HCC tissues with integrat- ed, disruptive and disappeared envelope in turn. There was no positive expression of MMP-2 and TIMP-2 in normal hepatic tissue. Conclusions The expression of MMP-2 and TIMP-2 could be important indexes in judging invasive and metastatic potency of HCC.