HPC脂质体的制备及抗HL-60细胞增殖作用的初探

The Preparation of Liposome by Using Hexadecylphosphocholine (HPC)and Its Cytotoxic Activity Assay on HL 60 Cells

采用十六烷基磷酸胆碱（ＨＰＣ）作为脂质体膜材，配以胆固醇和双十六烷基磷酸盐，反相蒸发法制备出ＨＰＣ脂质体，连续５周每周测定一次它对ＣＦ（ｃａｒｂｏｘｙｆｌｕｏｒｅｓｃｅｉｎ，羧基荧光素）的包封率，可知制备的脂质体在前２周内相当稳定，５周后包封率仅减少２４％，可满足实际应用的需要．冰冻蚀刻法测定脂质体的平均直径在５００ｎｍ左右，该直径的脂质体较适于和细胞发生相互作用且稳定性比小单层脂质体好．四氮唑（ｄｉｍｅｔｈｙｌｔｈｉａｚｏｌｄｉｐｈｅｈｙｌｔｅｔｒａｚｏｌｉｕｍｂｒｏ－ｍｉｄｅ，ＭＴＴ）分析可知，在脂质体浓度达１５μｍｏｌ／Ｌ，对ＨＬ－６０细胞的增殖具有抑制作用．在相同的脂浓度下，ＨＰＣ脂质体抑制ＨＬ－６０细胞生长比游离ＨＰＣ有较强的抑制细胞增殖作用，当ＨＰＣ浓度低于５μｍｏｌ／Ｌ时，细胞生长不受抑制．当ＨＰＣ浓度在１０μｍｏｌ／Ｌ时，ＨＰＣ脂质体表现出对细胞生长的抑制作用。

As a first step,the liposome forming properties of HPC were investigated,In the presence of cholesterol and dicetylphosphate,HPC formed liposomes with slow permeability for entrapped carboxyfluorescein.The encapsulation capacity(e) was about 79% and the liposomes were stable within 5 weeks.Vesicle size was estimated by electron microscopy and the mean diameter of liposomes 510±24 nm.The influence of HPC was investigated in vitro ,and HL 60 cells were incubated in the culture with free HPC or liposomal HPC,MTT(dimethylthiazol diphehyltetrazolium bromide)assay showed different cytotoxic effect between free HPC and liposomal HPC,whereas an enhanced cytotoxic effect was observed with liposomal HPC.