摘要
野生型苜蓿银纹夜蛾核型多角体病毒（ＡｃＮＰＶ）能感染棉铃虫细胞，不引起细胞凋亡，用ＬａｃＺ基因使ＡｃＮＰＶ凋亡抑制基因ｐ３５插入失活，得到缺陷病毒Ａｃｐ３５Ｚ能迅速引起棉铃虫细胞凋亡，但缺陷病毒本身不能复制。用ＡｃＮＰＶ即早期基因ＩＥ１启动子带动棉铃虫杆状病毒（ＨａＮＰＶ）ｐ３５基因在棉铃虫细胞中瞬时表达，能拯救ｐ３５基因失活病毒Ａｃｐ３５Ｚ复制。通过Ｘ－ｇａｌ显色反应和ｄｏｔＥＬＩＳＡ分别检测到了半乳糖苷酶的活性和ｐ３５基因的表达，证明了所克隆的ＨａＮＰＶｐ３５基因不仅是一个凋亡抑制基因，也是一个与杆状病毒复制相关的基因，它的瞬时表达能支持Ａｃｐ３５Ｚ在棉铃虫细胞中复制。
p35 gene from baculovirus is an antiapoptosis gene which can sustain virus multiplication when infected by baculovirus. Both p35 deleted and inserting inactivated AcNPV can not replicate in Sf cell lines and Ha cell line.It is almost the same in BmNPV.Acp35Z which generated from AcNPV inserted LacZ gene whthin p35 gene causes apoptosis in Ha cell line ,but the abortion replication can be rescued by transient expression of HaNPV p35 gene drived by AcNPV IE1 promotor.The result was reconfirmed with X-gal manifest and Dot-ELISA.
出处
《中国病毒学》
CSCD
1998年第3期251-256,共6页
Virologica Sinica
