缺血缺氧脑损伤大鼠IL-6和TNF-α的变化及其临床意义

Clinical Significance of Changes of Serum IL-6 and TNF-α Levels in Rat Models of Hypoxic-Ischemia Brain Injury

目的:探讨缺血缺氧脑损伤大鼠白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的动态变化及其临床意义。方法:90只Wistar大鼠随机分为对照组和实验组,再将实验组按造模后4h、8h、12h、24h和48h不同时点分为5个亚组。利用夹闭双侧颈总动脉,并进行缺氧的方法制备模型。以放射免疫分析(RIA)测定血清和脑组织中IL-6和TNF-α的变化。结果:造模后48h内,血清和脑组织中IL-6和TNF-α的水平呈动态变化。实验组中各亚组的两者含量均明显高于对照组(P<0.01或P<0.05)。血清和脑组织中两者分别在24h和12h时达到峰值。结论:缺血缺氧后不同时点IL-6和TNF-α的含量呈动态变化;两者可作为缺血缺氧后脑组织损伤程度及修复的临床指标。

Objective To explore the clinical significance of changes of serum interleukin-6（IL-6） and tumor necrosis factor alpha（TNF-α） levels in rat models of hypoxic-ischemia（HI） brain injury.Methods Seventy five rat HI brain injury nodels were prepared with bilateral occlusion of common carotid artery for 24rs followed 2hrs later by hypoxia（breathing 8% oxygen） for 2hrs.One fifth of the animals were sacrificed at 4h,8h,12h,24h and 48h later respectively,the serum and brain homogenate concentrations of IL-6 and TNF-α were determined with RIA and brain tissues were pathologically examined.Results The concentrations of IL-6 and TNF-α were dynamically changed within 48h in serum and brain homogenate.Peak values occurred at 24h with serum and at 12h with brain homogenate.Meanwhile,levels of both cytokines were significantly higher in the models than those in controls（P〈0.01 or P〈0.05）.Conclusion The concentrations of IL-6 and TNF-α were dynamically（sham operation only,15 animals） changed and might be regarded as the clinical markers of degree of HI brain injury.