用反转录-聚合酶链反应检测B组轮状病毒

Detection of Group B Rotavirus by RT PCR

轮状病毒（Ｒｏｔａｖｉｒｕｓ）是属于呼肠病毒科（Ｒｅｏｖｉｒｉｄａｅ）的双链ＲＮＡ（ｄｓＲＮＡ）病毒。至今已将轮状病毒分为七个组（Ａ～Ｇ）。已经发现的Ｂ组轮状病毒分别来自人、大鼠、牛、猪、羊。近十年来，通过轮状病毒的研究，轮状病毒Ｂ组已被公认为引起人...

A diagnostic assay was presented to detect group B rotavirus (GBRV) in fecal specimens with reverse transcription polymerase chain reaction (RT PCR). GBRV double strand RNAs (dsRNA) isolated from stool samples were reverse transcribed and amplified by PCR using two oligonucleotide primers which were derived from genomic segment 3 of the IDIR (intestinal disease of infants rat) strain of GBRV. This RT PCR assay permitted the sensitive and specific detection of a variety of GBRV in fecal specimens. Wild GBRV strains of lamb, kid were detected with these primer pairs by RT PCR. Moreover, RT PCR also permitted the detection of genomic RNA of lamb GBRV KB 63 strain which has been adapted to serial passage in cattle. The specific PCR products of 290 bp suggested that GBRV from lamb, kid and calf had identical sequences in genomic RNA; whereas the samples from fecal specimens containing group A rotaviruses (GARV), swine group B rotavirus (GBRV) and group C rotavirus (GCRV) or fecal specimens from uninfected controls gave negative reaction.