淋巴管透明质酸受体1和血管内皮生长因子受体3在胃癌组织中的表达

Expression of lymphatic vessel hyaluronan receptor-1 and vascular endothelial growth factor recep- tor-3 in gastric cancer

目的以淋巴管透明质酸受体1(LYVE-1)和血管内皮生长因子受体3(VEGFR-3)作为淋巴管特异性标志物,研究胃癌组织和癌周组织中淋巴管的生成表达情况。方法应用免疫组织化学和Real-time聚合酶链反应的方法检测62例胃癌、癌周组织中的LYVE-1和VEGFR-3蛋白及其mRNA表达情况,计算淋巴管的密度(LVD),检测淋巴管mRNA的相对含量。结果胃癌组织周边区淋巴管的密度高于胃癌组织中心区,而癌旁正常组织中很少见LYVE-1和VEGFR-3染色阳性的微淋巴管;胃癌组织周边区LVD和胃癌组织中LYVE-1和VEGFR-3mRNA含量与胃癌组织浸润深度、淋巴结转移、肿瘤分期及术后复发有关(P<0.05)。结论LYVE-1是一种特异性高于VEGFR-3的淋巴管内皮特异性标志物,淋巴管生成促进胃癌发展及淋巴结转移。

Objective To investigate lymphangiogenesis in paritumor and intratumor tissue of gastric cancer by detecting the expression of specific lymphatic endothelium marker of lymphatic vessel hyaluronan receptor-1（LYVE-1） and vascular endothelial growth factor receptor-3（VEGFR-3）. Methods LYVE-1,VEGFR-3 and their mRNA were examined in 62 gastric cancer tissue by routine immunohistochemical method and real time polymerase chain reaction respectively. Then the density of lymph vessel and the content of LYVE-1 and VEGFR-3 mRNA were calculated. Results The density of lymph vessel was higher in paritumor than intratumor tissue. The expression of LYVE-1 and VEGFR-3 protein could not be detected in normal tissue. The density of lymph vessel in paritumor tissue and the mRNA content of LYVE-1 and VEGFR-3 in cancer tissue were corre- lated with invasion depth, lymphnode metastasis, tumor stage and postoperational recurrence（P〈0.05）. Conclusions LYVE-1 is one kind of more specific lymphatic endothelium marker than VEGFR-3. Lymphangiogenesis can promote the progress and tymph node metastasis in gastric cancer.