LN和MMP-9表达与喉癌的相关性研究

CORRELATION STUDY BETWEEN LN、MMP-9 EXPRESSIONS AND LARYNGEAL CARCINOMA

目的研究层粘连蛋白(laminin,LN)和基质金属蛋白酶-9(MMP-9)在喉癌组织中的表达与其生物学行为关系以及它们之间的相关性。方法采用免疫组织化学SP法检测46例喉癌及30例正常喉咽黏膜中LN和MMP-9的表达,分析两者在有无淋巴结转移的癌组织中表达的差异及其相关性。结果LN和MMP-9在喉癌组织的阳性表达率(分别为87.2%和91.5%)明显高于正常喉组织(分别为9.1%和18.2%);LN和MMP-9在喉癌组织的阳性表达与有无淋巴结转移情况有关;LN和MMP-9的表达呈正相关性。结论LN和MMP-9在喉癌组织有阳性表达,表达的强度与喉癌的不同分化程度、临床分期、有无淋巴结转移情况有关,LN和MMP-9在喉癌中的表达有明显的相关性,表明两者可能在喉的发生发展及淋巴结转移过程中起协同作用。

Objective To investigate the expressions of laminin （LN） and matrix metalloproteinase-9 （MMP- 9） in laryngeal carcinoma and its relationship with the biological behavior in laryngeal carcinoma and to research the mechanism of carcinogenesis; we also analyze the correlation between LN and MMP-9. Methods The technology of immunohistochemistry SP was used to detect the expressions of LN and MMP-9 in the 46 cases of laryngeal carcinoma and those in 30 cases normal mucosa respectively. In addition, several clinic pathological features were analyzed. Results The positive expression rates of LN and MMP-9 in laryngeal carcinoma were 73. 9%and 70% respectively,higher than those of being 23.3% and 16.7% in normal laryngeal tissues. In laryngeal carcinoma, the intensive expression rates of LN and MMP-9 had a positive relationship with the situation of cervical lymph node matastasis. Significant correlation was found between the expression of LN and that of MMP-9 in laryngeal carcinoma. Conclusion LN and MMP-9 show positive expressions in laryngeal carcinoma. The degree of expressions of LN and MMP-9 have relevance to the degree of differentiation, clinical stage and cervical lymph node matastasis of laryngeal carcinoma. The expressions of LN and MMP-9 in laryngeal carcinoma were positively correlated, playing synergistic effect during the process of occurrence and metastasis of laryngeal carcinoma.