人肝细胞生长因子cDNA克隆在CHO细胞中的表达

Expression of Human Hepatocyte Growth Factor with Chinese Hamster Ovary(CHO) Cells

肝细胞生长因子是一种由α、β链组成的杂合二聚体糖蛋白，能促进肝细胞、多种上皮细胞、内皮细胞和神经胶质细胞的有丝分裂，并对多种肿瘤细胞具有细胞毒性作用或者抑制其生长［１～４］，其作用无种属特异性，如人肝细胞生长因子能促进大鼠肝细胞增殖［５］。由于天然Ｈ...

The cDNA gene encoding human hepatocyte growth factor(hHGF)was inserted into pcDNA3 plasmid and obtained pcDNA3/hHGF expression plasmid.The pcDNA3/hHGF plasmid was transfected into CHO cells with calcium phosphate DNA precipitate method.The expression clones was obtained by G418 selection.The culture supernatant from the positive clones could stimulating the mitosis of primary culture of rat hepatocytes and inhibiting proliferation of HepG2 cells were observed.