利用组织培养技术选育玉米抗小叶斑病突变体

Selection of Resistant Mutants for Helminthosporium maydis via Tissue Culture in Maize

大叶斑病和小叶斑病是玉米最严重的病害，选育抗大、小叶斑病优良自交系及单交种是提高玉米产量的重要措施。Ｇｅｎｇｅｎｂａｃｈ等以玉米Ａ６１９ｃｍｓＴ愈伤组织为材料，筛选出对小叶斑病毒素具有抗性的愈伤组织与再生植株［１，２］，开辟了玉米抗病育种的新途径。...

Embryogenic calli derived from the commercial selfline Huangzhaosi of maize were transferred to medium supplemented with toxin from Helminthosporium maydis for the selection of resistant cells. After three selections, toxin resistant calli were produced. The toxin showed obviously effects on the proliferation and differentiation of callus cells. On the medium added the toxin, plantlets were regenerated from toxin resistant calli. The progenies of one regenerated plant showed significantly stronger resistance than Huanghzaosi, It was concluded that the toxin resistant character could be stably inherited by the analyses of data from backross plants.