HPLC法测定强力脑清素片中紫丁香苷含量

Determination of Qiangli Naoqing Su tablets syringin content by HPLC

目的建立高效液相色谱(HPLC)法测定强力脑清素片中紫丁香苷含量的方法。方法采用HPLC法,以十八烷基硅烷键合硅胶为填充剂,Agilentzorbasil4.6mm×250mm,5μm,甲醇-水(20:80)为流动相,检测波长为265nm,流速1.0ml/min。结果紫丁香苷在0.080～0.800μg/ml范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为97.7%,相对标准偏差值(RSD)=1.25%。结论本方法测定结果准确、操作方法简便、分离度好,可用于强力脑清素片质量控制提供参考。

Objective To establish an HPLC method for determination of syringin content in Qiangli Naoqing Su tablets. Methods HPLC was adopted, wtih Agilent zorbasil C18 colum 4.6 mm × 250 mm, 5 μm, methanol-water （20： 80） as mobile phase. The wavelength used for detection was 265 nm. The flow rate was 1.0 ml/min. Results The liner range of syringin was 0. 080 - 0. 800 μg （ r = 0.9999 ）. The average recovery of astilbin was 97.7%, RSD = 1.25%. Conclusion This method is accurate and suitable, and can be used for the quality control of Qiangli Naoqing Su tablets.