治疗用武汉抗CD3单克隆抗体(WuT_3)的致突变性研究

Studies on Mutagenicity of Wuhan Monoclonal Antibody against CD3 (WuT_3) for Curing

观察了武汉抗ＣＤ３单克隆抗体（简称ＷｕＴ３）对组氨酸缺陷型鼠伤寒沙门氏菌ＴＡ９７、ＴＡ９８、ＴＡ１００及ＴＡ１０２菌株的回复突变作用。结果显示在５～５０００μｇ／皿的剂量范围内，ＷｕＴ３所致的诱发回复突变菌落数与自发回复突变菌落数之比ＭＲ（Ｒｔ／Ｒｃ），无论加大鼠肝匀浆，辅酶Ⅱ及葡萄糖６－磷酸（Ｓ－９混合液）或不加Ｓ－９混合液，均不超过２。同时观察了ＷｕＴ３对小鼠骨髓细胞微核率及对人外周血淋巴细胞染色体畸变率的影响，结果显示小白鼠ｉｖＷｕＴ３，每日一次，连续２日，在２５～１００ｍｇ·ｋｇ－１范围内，ＷｕＴ３各剂量组的微核细胞率与溶剂对照组相比均无显著性差异（Ｐ＞０．０５）。而环磷酰胺（ＣＰ）阳性组与溶剂对照组相比有极显著性差异（Ｐ＜０．０１）。ＷｕＴ３在２５～２５０μｇ／瓶的剂量范围内，各剂量组的染色体畸变细胞率与溶剂对照组相比无显著性差异（Ｐ＞０．０５），而ＣＰ组与溶剂对照组相比有极显著性差异（Ｐ＜０．０１）。

The effects of Wuhan monoclonal antibody against CD3 (WuT3) for curing on the revertant mutation of histidinerequiring Salmonella typhimurium TA97、TA98、TA100 and TA102 strains were observed.The results showed that all the ratios of induced revertant mutation clones to spontaneous revertant mutation clones were under 2,with S-9 and without S-9,in the concentration of 5～5000 μg/plate for WuT3.The frequencies of micronucleated cells induced by WuT3 in mice bone marrow cells were also observed.The mice were treated with 25～100 mg·Kg-1 of WuT3,iv,once a day for 2d,the results showed the frequencies of micronucleated cells in each dosage group of WuT3 were not significantly higher than that in negative control (P>0.05) and the frequency in cyclophosmide (CP) group was significantly higher than that in negative control (P<0.01).The effects of WuT3 on the chromosomal aberration of human peripheral blood lymphocytes were studied.It was found that in the concentration of 25～250 μg/bottle for WuT3,the frequencies of chromosomal aberration cells in each dosage group for WuT3 were not significantly difference when compared with that in negative control (P>0.05),and CP group was significantly higher than negative control (P<0.01). Above all,the results of these tests indicated:there was no mutagenicity with WuT3.