摘要
观察了武汉抗CD3单克隆抗体(简称WuT3)对组氨酸缺陷型鼠伤寒沙门氏菌TA97、TA98、TA100及TA102菌株的回复突变作用。结果显示在5~5000μg/皿的剂量范围内,WuT3所致的诱发回复突变菌落数与自发回复突变菌落数之比MR(Rt/Rc),无论加大鼠肝匀浆,辅酶Ⅱ及葡萄糖6-磷酸(S-9混合液)或不加S-9混合液,均不超过2。同时观察了WuT3对小鼠骨髓细胞微核率及对人外周血淋巴细胞染色体畸变率的影响,结果显示小白鼠ivWuT3,每日一次,连续2日,在25~100mg·kg-1范围内,WuT3各剂量组的微核细胞率与溶剂对照组相比均无显著性差异(P>0.05)。而环磷酰胺(CP)阳性组与溶剂对照组相比有极显著性差异(P<0.01)。WuT3在25~250μg/瓶的剂量范围内,各剂量组的染色体畸变细胞率与溶剂对照组相比无显著性差异(P>0.05),而CP组与溶剂对照组相比有极显著性差异(P<0.01)。
The effects of Wuhan monoclonal antibody against CD3 (WuT3) for curing on the revertant mutation of histidinerequiring Salmonella typhimurium TA97、TA98、TA100 and TA102 strains were observed.The results showed that all the ratios of induced revertant mutation clones to spontaneous revertant mutation clones were under 2,with S-9 and without S-9,in the concentration of 5~5000 μg/plate for WuT3.The frequencies of micronucleated cells induced by WuT3 in mice bone marrow cells were also observed.The mice were treated with 25~100 mg·Kg-1 of WuT3,iv,once a day for 2d,the results showed the frequencies of micronucleated cells in each dosage group of WuT3 were not significantly higher than that in negative control (P>0.05) and the frequency in cyclophosmide (CP) group was significantly higher than that in negative control (P<0.01).The effects of WuT3 on the chromosomal aberration of human peripheral blood lymphocytes were studied.It was found that in the concentration of 25~250 μg/bottle for WuT3,the frequencies of chromosomal aberration cells in each dosage group for WuT3 were not significantly difference when compared with that in negative control (P>0.05),and CP group was significantly higher than negative control (P<0.01). Above all,the results of these tests indicated:there was no mutagenicity with WuT3.
出处
《微生物学免疫学进展》
1998年第3期6-9,共4页
Progress In Microbiology and Immunology
关键词
致突变性
抗CD3
单克隆抗体
微核率
WuT3
Monoclonal antibody to CD3 Mutagenicity Revertant mutation Chromosomal aberration Micronucleus