光动力疗法对动物肿瘤生长的影响及机制研究

The Effect of Photodynamic Therapy of the Growth of Tumor and Research of Mechanism in Rats

本实验选用经腹股沟皮下接种Ｗ２５６瘤细胞株形成实验性荷瘤大鼠４０只，采用光动力学疗法对患有移植瘤大鼠进行治疗，并同时检测血浆、肝脏及瘤组织中超氧化物歧化酶的活力及氧自由基代谢产物丙二醛含量。结果如下：（１）大鼠输入经紫外光照射之血后，其肿瘤生长速度明显减慢（Ｐ＜０．０５），大鼠输入注有血卟啉衍生物的血后，其肿瘤生长亦显著减慢（Ｐ＜０．０１）；大鼠输入经紫外光照射并含有血卟啉衍生物之血后，其肿瘤生长速度比其它组均显著减慢（Ｐ＜０．０１）；（２）光照射组大鼠在肿瘤生长减慢同时，其血浆及瘤组织中Ｔ—ＳＯＤ活力明显升高；ＭＤＡ含量显著降低（Ｐ＜０．０５—０．０１）；而血卟啉组大鼠其血浆和瘤组织中ＴＳＯＤ活力明显降低，ＭＤＡ含量显著升高（Ｐ＜０．０１）；光照射加血卟啉组大鼠，在肿瘤生长减慢同时，其血浆、肝脏及瘤组织中ＴＳＯＤ活力较其它组均显著降低，ＭＤＡ含量显著升高（Ｐ＜０．０１），提示：给大鼠输入经紫外光照射的血或含有血卟啉的血均对肿瘤生长有抑制作用，而且紫外光照射与注入血卟啉相结合对遏制肿瘤生长效果最佳，因体内ＴＳＯＤ及ＭＤＡ的改变，故推测该光动力学疗法对肿瘤治疗作用机制之一可能是通过产生单态氧而杀伤瘤细胞的。

rats were implanted W256 tumor cells by subcutaneous vaccination at groin. Then, the rats were therapied by means of photodynamic therapy (PDT) and radiotherapy enhanced densitivity. After the end of experiment, the contents of superxides dismutase(SOD) and MDA were detected. The results were as follows:(1) The growth of tumor was significantly slow after the rats were transfused blood with ultraviolet radiation or with hematoporphyrin derivative(HPD) or with both ultraviolet radiation and HPD(P<0 050.01).(2) The activity of TSOD was obviously increased and the contents of MDA were decreased in plasma and while the growth of tumor was significantly slow in the ultraviolet radiation group(P<0 050.01). But, the activity of TSOD was weakened and the contents of MDA were increased in HPD group(P<0 01) or ultraviolet radiation and HPD group(P<0 01). The results indicate that the growth of tumor was inhibited by means of transfusion blood with ultraviolet radiation of HPD, but the effect of inhibition was the most remarkable in ultraviolet radiation and HPD group, The one of mechanism of PDT treatment tumor may be kill tumor cells by making monomorphic oxygen.