异搏定拮抗α-石英细胞毒性作用的研究

Research of Antagonism of Verapamil to the Cytotoxicity Induced by α Quartz.

借助于单细胞阳离子测定系统等手段，研究了不同细胞外钙浓度下异搏定作用后，细胞存活率与细胞内游离钙浓度（［Ｃａ２＋］ｉ）的变化关系．同时还研究了异搏定对实验性硅肺大鼠模型的作用．结果表明：由α石英引起的肺泡巨噬细胞内钙升高是由于大量细胞外钙内流，造成细胞内钙超载，引起细胞死亡，并进一步导致硅肺形成．异搏定可以抑制细胞内钙增加，降低细胞的死亡．动物模型实验结果还表明异搏定对硅肺的发生有明显的抑制作用．

By use of the AR CM MIC system and other methods, the relationship between cellular viability of alveolar macrophages and the change of intracellular Ca 2+ concentration([Ca 2+ ] i) after the action of verapamil was investigated under different extracellular calcium concentrations. And the research about the effect of verapamil on experimental silicosis rats were conducted. The results showed: the [Ca 2+ ] i rise induced by α quartz was caused by enormous extracellular Ca 2+ influx and the overloading of the intracellular Ca 2+ , which directly led to the death of alveolar macrophages and further the formation of silicosis. Verapamil could inhibit the [Ca 2+ ] i rise, decline the death of cells. The results of the research about experimental silicosis indicated: verapamil has apparent inhibitory effect on the starting of silicosis. It suggested that verapamil appears to be a prevention and cure drug of silicosis.