蓝藻叶绿素蛋白复合体的分离研究

Studies on Separation about Chl Protein Complexes of Blue green Algae.

蓝藻类囊体膜用声波超时处理，然后在４℃下用低浓度的ＬＤＳ增溶，并经改进的ＳＤＳ聚丙烯酰胺凝胶电泳后被分离成１５条绿色的带．其中ＣＰａ１～ＣＰａ６有着相似的吸收光谱．这６个组分的低温荧光光谱也很相似，其荧光发射光谱的发射峰都位于６８５ｎｍ处，表明它们都属于光系统Ⅱ叶绿素ａ蛋白复合体．

When the thylakoid membranes of blue green algae broken over a longer time than usual by sonication were solubilized with low concentration of LDS and subjected to discontinuous SDS PAGE improved at 4℃,15 containing chl bands for the first time was resolves . CPa1 CPa6 of them had similar absorption spectra. The fluorescence spectra of CPas at 77 K were very similar too,all having a emission peaks at 685 nm. It is concluded that 6 CPas were chl a protein complexes of PSⅡ . The resolved capability to PSⅡ of the new system were 3 times of the popular ones.