摘要
本文介绍了微球菌（Micrococcussp）1504胞外脂肪酶的代谢调节模式。发现在发酵培养基中，通过补加长链脂肪酸及其油酯，能提高脂肪酶的生产水平，而中、短链脂肪酸及其酯抑制产酶能力；高浓度的葡萄糖抑制产酶能力；补加不同浓度的橄揽油，适宜补加时间也应不同。水洗细胞脂肪酶的合成受橄榄油和油酸诱导，受葡萄糖和甘油阻遏，并发现脂肪酶的分解代谢阻遏主要发生在转录水平上。
The regulation of extracellular lipase synthesis from Micrococcus sp. 1504 was studied.Addition of long chain fatty acid and acyl esters to growing shake-flask cultUres, enhanced thelevel of lipase activity produced. but the effect of middie or short chain fatty acid and acyl esterswas opposite. High content of glucose ichibited the lipase production olive oil enhanced the lipaseproduction. The content of olive oil by added was change4 the optimum bine when olive oil wasadded should changed. Synthesis of lipase activity by washed cells was induced by olive oil andoleic act4 repressed by glucose and glycerol. It is suggested that catabolite repression of lipaseformation in Micrococcus sp. 1504 was mainly regulated by tlanscriptional control.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第4期202-205,共4页
Microbiology China
关键词
脂肪酶
诱导
阻遏
微球菌
Lipase, Induction, Repression, Micrococcus sp. 1504
