金针菇FV088菌株液体培养工艺的研究

STUDIES ON LIQUID STATE CULTURE CONDITIONS OF FLAMMULINA VELUTIPES FV088

通过单因子试验统计分析，优化筛选了适于金针菇（Flammulinavelutipes）FV088的适宜培养基和摇瓶培养条件，结果表明，其适宜的液体培养基组成为：5.0％玉米粉，2．0％鼓皮，0．l％KH2PO4，0．05％MgSO4·7H2O，10μgVB1／100ml，50μgVB2／100ml；适宜的摇瓶培养条件为：培养基的起始pH6．0～7．0，500ml摇瓶装量为150ml，接种量为10%，培养温度25℃，摇床转速为120r／min，菌丝干收率3．9g／100ml。

Using analyticla technic of single factor test statistics, a suitable medium and shaking culture conditions for Flammulina velutipes 088 have been selected. Experimental results showed that the composition of fermentation medium was as follow: 5.0% maize powder, 2.0% wheat bran, 0.1%KH2 Po4, 0.05%MgSo4· 7H2O, 10μgVB1 / 100ml, 50μgVB2 / 100ml; The suitable culture conditions were as follows: PH6.0～7.0,150ml medium in size 500ml flask. inoculation 10%, temperature 25℃, stirring speed 120r / min. The mycelical dry weight was 3.9g / 100ml.