少孢根霉RT-3胞外产超氧化物歧化酶的研究

STUDY ON SECRETION AND PRODUCTION OF SUPEROXIDE DISMUTASE OF RHIZOPUS OLIGOSPORUS

利用少抱根霉RT－3行固态发酵，从培养物中可以直接拍提得到SOD。固体发酵培养物发酵前后的酶活分析表明少抱根霉RT－3可向胞外分泌SOD。酶抽提液中含有Cu、Zn型和Mh型SOD。对其初步纯化所得粗酶制剂比活力为444．8u／mg。酶的紫外吸收峰在258nnl。酶在60℃以下较稳定，活性稳定pH为5．5～9．5。

Superoxide Dismutase (SOD) could be extracted from solid fermenting culture of Rhizopus oligosporus RT-3. Crude extract of SOD from the culture contained Cu, Zn-SOD and Mn-SOD. Specific activity of orude enzyme prepaation of SOD by preliminary purification was 444.8u/ mg. Ultraviolet absorption peak of this enzyme was 258nm. Stable pH and temperature for enzyme are 60℃ and 5.5～9.5.