摘要
从土样中分离筛选到一株脂肪酸菌株──毛霉（Mucorsp．）M,其优化后的培养基组成（％）：黄豆粉40、蔗糖0．5、橄榄油10、硫酸铵0．l、磷酸氢二钾0．2、硫酸镁O.01、pH自然。产酶最适条件：初始pH6.5、培养温度28℃、培养周期96h。该酶最适作用温度50℃、最适pH80、pH稳定范围为7．0～10.0，Fe2+、Ca2+、Mg2+、K+对酶有激活作用。
A Fungal strain (Mucor sp) M, prepucing lipase was screened from oilysoil, The optimized medium consists of (%): soybean flour 4.0, sucrose 0.5, olive oil.0, (NH4)2SO40.l,K=HPO40.2, MgSO40.0l, PH6.5. The optimal conditions for preducing lipase: PH6.5, 28℃, 96h.Tihs lipase has an optimum temperature of 50℃, an optimum PH of 8.0. It is stable in the PH range of 7.0～10.0. Metal ions, such as Fe2+. Ca2+. Mg2+、 K2+ can activate this enzyme.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第5期274-277,共4页
Microbiology China
关键词
毛霉
产酶条件
酶学性质
脂肪酶
Mucor sp., Condition for producing lipase, Properties of lipase
