原发性胆囊癌中P185和PTEN的表达及其与临床病理的相关性研究

Relationship between the expressions of P185 as well as PTEN protein and their clinicopathologic significance in primary gallbladder carcinoma

目的探讨P185和PTEN蛋白在原发性胆囊癌组织中的表达与癌组织类型、病理分级和转移状况的关系,以及P185与PTEN表达之间的相关性。方法应用免疫组化方法检测胆囊腺癌52例、胆囊腺瘤20例和慢性胆囊炎20例组织中P185和PTEN表达水平。结果在胆囊腺癌、胆囊腺瘤和慢性胆囊炎中,P185表达阳性率分别为61.5%(32/52)、20%(4/20)和0(0/20),PTEN表达阳性率分别为42.3%(22/52)、80.0%(16/20)和100%(20/20),组间比较差异均有统计学意义(P<0.05)。P185高表达和PTEN低表达与胆囊腺癌的分化程度、浆膜浸润和转移密切相关(P<0.05)。P185表达与PTEN蛋白表达呈负相关(P<0.05)。结论P185和PTEN表达与胆囊腺癌浸润转移密切相关。检测P185和PTEN蛋白表达可作为判断胆囊腺癌预后的客观指标。

Objective To investigate the relationship between P185 and PTEN protein expression and their histological types,pathological grading and metastasis. Methods Immunohistochemical method was used to detect the expression of P185 and PTEN protein in 52 cases of gallbladder carcinoma,20 cases of gallbladder adenoma and 20 cases of chromic cholecystis. Results The expression rates of P185 in gallbladder carcinoma,gallbladder adenoma and chromic cholecystis were 61.5%（32/52）,20%（4/20） and 0（0/20） respectively. The expression rates of PTEN in gallbladder carcinoma,gallbladder adenoma and chromic cholecystis were 42.3%（22/52）,80.0% （6/20） and 100%（20/20） respectively. There were significant differences among groups（P〈0.05）. The high expression of P185 and the low expression of PTEN were positively correlated with the pathological grading,serosa infiltration and metastasis in gallbladder carcinoma（P〈0.05）. The expression of P185 was negatively correlated wth PTEN expression in gallbladder carcinoma（P〈0.05）. Conclusion The expression of P185 and PTEN is closely related with the invasion and metastasis of gallbladder carcinoma,and the detection of the expression of P185 and PTEN protein can be used as the prognostic indicators in gallbladder carcinoma.