替米沙坦对2型糖尿病大鼠心肌病变的干预研究

Effects of telmisartan on diabetic cardiomyopathy in rats with type 2 diabetes

目的观察2型糖尿病大鼠心肌超微结构和心功能变化及替米沙坦对其的影响。方法雄性SD大鼠40只,单纯随机分为正常组(N组)、糖尿病模型组(M组)、替米沙坦干预组(T组)。M、T组大鼠高糖高脂饲料喂养4周后以25mg/kg链脲佐菌素(STZ)尾静脉1次性注射,建立2型糖尿病大鼠模型。N组常规饲料喂养4周后以等剂量的枸橼酸盐尾静脉注射。所有大鼠均以原饲料喂养。STZ注射后1周,T组给予替米沙坦8.34mg·kg-1.d-1,N、M组给予等量水灌胃,给药12周末结束实验,取血测空腹血糖(FBG)、空腹胰岛素(FINS)、总胆固醇(TC)、三酰甘油(TG);制备电镜切片,监测心功能。结果M、T组血INS、TG均显著高于N组(P<0.01),T组INS、TG显著低于M组(P<0.01)。结论替米沙坦能够改善糖尿病心肌病,机制可能与降低INS、TG有关。

Objective To observe the effects of telmisartan on diabetic cardiomyopathy in rats with type 2 diabetes. Methods 40 male SD rats were randomly divided into normal group （group N） and diabetes model group （group M）,telmisartan intervention group （group T）. The rats in group M and group T were injected via the tail vein with low-dose streptozotocin（STZ,25 mg/kg） after 4-week feed with high calorie diet;the rats in group N were injected via the tail vein with the same volume citric acid buffer after 4week feed with common diet. One week after injection with STZ,the rats in group T were given with telmisartan 8.34 mg·kg^-1·d^-1,however,the rats in group N and group M were given with the same volume water. The experiment ended until 12 weeks after giving telmisartan. The blood samples were got to measure the levels of blood glucose,insulin,cholesterol,triglyceride,and the heart function was detected,and the electron microscope slices of cardiomyopathy were prepared. Results The levels of INS and TG in group M and group T were significantly higher than those of group N（P〈0.01）,however,the levels of INS and TG in group T were significantly lower than those in group M （P〈0.01）. Conclusion The telmisartan can improve diabetic cardiomyopathy,which may be related with the decrease of the levels of INS and TG.