芦荟大黄素对人膀胱癌细胞FasL基因表达和侵袭能力的影响

Influence of Aloe-emodin on Fas Ligand Gene Expression and Invasive Ability of Human Bladder Cancer Cell

目的:在体外通过细胞培养,初步研究芦荟大黄素对人膀胱癌细胞系T24细胞FasLmRNA表达及对其侵袭能力的影响,为中药抗肿瘤提供实验依据。方法:根据MTT法得到芦荟大黄素对T24细胞的半数有效抑制浓度(IC50),确定药物作用浓度。T24细胞分别经芦荟大黄素不同浓度(0.5IC50、IC50)作用后,应用逆转录-聚合酶链反应(RT-PCR)法检测芦荟大黄素作用前后人膀胱癌细胞系T24细胞FasL mRNA的变化;应用Transwell细胞侵袭试验检测芦荟大黄素对T24细胞侵袭能力的影响。结果:芦荟大黄素处理后较处理前T24细胞FasL mRNA表达水平均明显低于对照组(P<0.05);而且FasL mRNA表达水平随芦荟大黄素作用浓度增加显著下调,芦荟大黄素不同浓度组比较均有显著性差异(P<0.05);随芦荟大黄素作用浓度升高,T24细胞侵袭能力明显减弱,不同浓度组比较均有显著性差异(P<0.05)。结论:芦荟大黄素在一定时间内均可下调人膀胱癌细胞系T24细胞FasLmRNA的表达,而且这种下调作用在一定范围内呈剂量依赖性,可使膀胱癌细胞的侵袭能力降低。

Objective：To study the influence of Aloe-emodin on Fas Ligand mRNA expression of human Bladder Cancer Cell line T24 and invasive ability with cell culture. Methods：The inhibition concentration 50%（IC50） of Aloe-emodin was detected with MTT assay. Different concentrations（0.5IC50,IC50） of Aloe-emodin was used in the cultured human Bladder Cancer Cell line T24. The changes of Fas Ligand mRNA were detected by the reverse transcription polymerase chain reaction method（RT-PCR） and invasive ability of T24 cells was measured by Transwell-ECM. Results：RT-PCR method revealed that the higher concentration Aloe-emodin upregulated Fas Ligand mRNA expression on T24 cells（p 0.05） ;Transwell-ECM showed that the higher concentration Aloe-emodin increased apoptosis rate of lymphocyte induced by T24 cells（p〈0.05） . The comparisons with different concentrations teams of Aloe-emodin have statistical significance（p〈0.05） . Conclusion：Aloe-emodin can downregulate functional Fas Ligand mRNA expression of human bladder cancer cell line T24,which presents the dosage dependence and reduces the invasiveness of bladder cancer cell.