COPD大鼠肺组织中Muc5ac和IL-13的相关性研究

The Relative Study of Muc5ac and IL-13 in Mouce Lungs of COPD

目的:研究慢性阻塞性肺病(COPD)大鼠气道粘蛋白Muc5ac和白介素13(IL-13)的表达及其相关性。方法:20只wistar雄性大鼠随机分为对照组和COPD组,正常组8只,COPD组12只,分别用免疫组化法测定肺组织Muc5ac和IL-13的表达。结果:Muc5ac和IL-13蛋白表达主要位于气道上皮细胞,细胞胞浆呈棕黄色。COPD组大鼠肺组织IL-13和Muc5ac的表达均高于正常组(P均<0.05)。结论:IL-13是引起粘液分泌的重要因子,它可能上调Muc5ac的表达。

Objective：To study Muc5ac and interleukin 13（IL-13） expression and relativity in mouce lungs of COPD. Methods：Twenty male wistar mice were randomly divided into control group and COPD group,eight mice control group and twelve mice COPD group. Muc5ac and IL-13 expression were detected by immunohistochemical method,respectively. Results：Both Muc5ac and IL-13 positive products were located in the cytoplasm of airway epithelial cells. Compared with control group,IL-13 could increase Muc5ac（all P〈0.05） . Conclusion：IL-13 is a critical cytokine inducing airway mucus overproduction,and may increase Muc5ac expression.