雾化吸人布地奈德对肺纤维化大鼠支气管肺泡灌洗液中肿瘤坏死因子α及血小板衍生生长因子的影响

Effects of inhaled budesonide on tumor necrosis factor-α and platelet-derived growth factor in bronchoalveolar lavage fluid of pulmonary fibrosis rats

目的探讨雾化吸入布地奈德对博莱霉素致肺纤维化大鼠支气管肺泡灌洗液（BALF）中肿瘤坏死因子α（TNF-α）及血小板衍生生长因子（PDGF）的影响。方法45只Wistar雌性大鼠随机分为生理盐水对照组（CG组）、肺纤维化模型组（MG组）、布地奈德干预组（BG组），每组15只。利用博莱霉素建立肺纤维化模型，24h后给予干预药物，分别于第7天、第14天、第28天处死动物，收集肺组织作切片行HE及Masson染色，观察其病理变化，判断肺组织肺泡炎及肺纤维化程度，测定肺组织羟脯氨酸含量，并测定BALF中TNF-α及PDGF的含量。结果BG组第7天、第14天、第28天肺组织羟脯氨酸含量低于MG组（P〈0．05）。BG组在早期（第7天、第14天）BALF中TNF—α及PDGF含量低于MG组（P〈0．05）。结论雾化吸人布地奈德对博莱霉素诱导的肺纤维化大鼠在早期具有较强的抗纤维化效应，机制可能为通过抑制细胞因子TNF-α及PDGF含量的表达，从而有效阻止肺纤维化的进展。

Objective To observe the effects of inhaled budesonide on tumor necrosis factor-α （TNF-α） and platelet-derived growth factor （PDGF） in bronchoalveolar lavage fluid （BALF） of bleomycininduced pulmonary fibrosis rats. Methods Forty-five Wistar female rats were randomly divided into control group（CG）, pulmonary fibrosis model group （MG） and budesonide intervention group （BG）, each group including 15 rats. The model of pulmonary fibrosis was established by a single intratracheal instillation of bleomycin. After 24 hours lboth CG and MG were aerosolized 0.9% NaC1 solution, while BG was aerosolized budesonide. On the 7th, 14th and 28th day after treatment, five rats of each group were sacrificed,respectively, and their lungs were harvested to examine pathological change, hydroxyproline content in lung tissue. Bronchoalveolar lavage was used to detect the concentration of TNF-αand PDGF in BALF. Results The hydroxyproline concentration in lung tissue of BG was lower than that in MG on the 7th, 14th and 28th day（ P 〈0. 05）. The levels of TNF-α and PDGF in BALF of BG were lower than those in MG in early stage（7-14 days） （ P 〈0.05）. Conclusion Inhaled budesonide, especially in early stage. could ameliorate the formation of bleomycin-induced pulmonary fibrosis through inhibiting the release of TNF-α and PDGF.