土霉素在红鳍东方鲀体内的残留及休药期研究

Residues and withdrawal period of oxytetracycline in globefish Fugu rubripes Temmincket schlegel

在14±2℃水温条件下,连续5d对红鳍东方鲀口灌剂量为100mg/kg的土霉素,采用高效液相色谱法测定了停药后血清、肌肉、肝脏组织中的药物含量、消除速率常数和消除半衰期,提出了该温度下的休药期。研究表明,在停药2d后红鳍东方鲀血清和肌肉中的药物浓度达到峰值,分别为1.092μg/ml和0.806μg/g;停药3d后肝脏内土霉素浓度达到峰值1.229μg/g,土霉素在血清、肌肉和肝脏中的消除半衰期分别为23.8、22.4和26.8d;土霉素在红鳍东方鲀肌肉组织中降到0.1μg/g残留限量的时间为58d,降到0.05μg/g残留限量的时间为81d。

Residues and withdrawal periods of oxytetracycline （OTC） was evaluated by HPLC in globefish Fugu rubripes following multi-oral administration of 100 mg/kg weight body per day for 5 days at 14±2℃. The result showed that the highest concentrations of OTC in blood and muscle were found in 2 days after the last administration, which were 1. 092μg/ml and 0. 806μg/g, respectively. While the highest level found in liver was 1. 229μg/g, 3 days post-administration. It was revealed that the elimination half-lives of OTC in blood, muscle and liver were 23.8, 22.4 and 26.8 days respectively, indicating that the elimination rate of OTC in globefish was very low. OTC concentrations in the muscle of globefish fell below the 0. 1μg/g and 0.05μg/g residue limits at day 58 and day 81 post-administration, respectively.