正交试验法优选鬼臼毒素新型脂质体的制备工艺

Study on the optimum preparation condition of the new type podophyllotoxin liposome with orthogonal design

确立鬼臼毒素新型脂质体的最佳制备工艺。采用正交设计方法对最佳制备工艺进行实验,以氢化磷脂与胆固醇比例、氢化磷脂与PAMAM dendrimer G3.5脂质小球的比例、氢化磷脂与鬼臼毒素的比例、旋转蒸发温度为4个因素,包封率为考察指标,筛选出鬼臼毒素新型脂质体最佳制备工艺。优选出鬼臼毒素新型脂质体的制备工艺为氢化磷脂∶胆固醇=20∶3.26,氢化磷脂∶PAMA Mdendrimer G3.5脂质小球=20∶0.5,氢化磷脂∶鬼臼毒素=20∶1.5,旋转蒸发温度为45℃。所选工艺制备的成品符合脂质体的质量标准。

To select the optimum condition of the new type podophyllotoxin of the new type podophyllotoxin liposome preparation was performed based The entrapment efficiency of the new type podophyllotoxin liposome were liposome preparation. The optimization on the orthogonal experimental design. taken as the index and the influence of factors such as the proportion of hydrogenated phospholipid and Cholesterol, the proportion of hydrogenated phospholipid and PAMAM dendrimer G3.5 lipid globule, the proportion of hydrogenated phospholipid and podophyllotoxin and the temperature of rotary evaporation. The best formulation of the new type podophyllotoxin liposome were the proportion of hydrogenated phospholipid and Cholesterol was 20 ： 3.26, the proportion of hydrogenated phospholipid and PAMAM dendrimer G3.5 lipid globule was 20 ： 0. 5, the proportion of hydrogenated phospholipid and podophyllotoxin was 20 ： 1.5 and the temperature of rotary evaporation was 45℃. The new type podophyllotoxin liposome designed according to orthogonal experiment was coincidenced with the requirements in aspect of the liposome.