Tesmin基因在小鼠睾丸中的表达分析

The expression of Tesmin in the development of mouse testicle

目的研究Tesmin基因的表达特点。方法将4d、9d、18d、35d、54d和6月龄小鼠睾丸组织提取RNA,通过RT-PCR分析差异表达基因Tesmin在小鼠睾丸不同发育阶段中的表达。结果基因芯片结果显示4d、9d、18d、35d、54d和6月龄小鼠睾丸中分别是31.4(A)、34(A),1292(P)、865.6(P)、977.2(P)和830.7(P),代表Tesmin基因在4d,9d小鼠中无表达,1 8d龄后开始高表达,RT-PCR结果表明小鼠Tesmin基因在小鼠9d龄及之前的睾丸中没有表达,在18d龄后睾丸中开始高表达,与基因芯片分析结果相一致。结论Tesmin基因为小鼠年龄依赖性表达基因,小鼠Tesmin基因的表达与小鼠精子发生有很强的一致性,推测该基因可能在精子发生中起重要作用。

Objective To investigate the expression of Tesmin in the development of mouse testicle. Methods Testes samples from Balb/c mice at different postnatal days （days 4, 9, 18, 35, 54, and 6 months） were collected. Differential genes in the development of mouse testicle were screened by mouse whole genome 430 2.0 Array （Affymetrix Inc.） chip. The interested gene Tesmin was further confirmed by RT-PCR. Results The expression of Tesmin was gradually upregulated following with developmental of mouse testis. The hybridization signal intensities of the tested testis on d 4, d 9, d 18, d 35, d54, and 6 months of postnatal were 31.4 （Absent expression, A）, 34 （A）, 1292 （Present expression, P）, 856.6（P）, 977.2 （P） and 830.7 （P） respectively. The expression of Tesmin was detected on d 18, d 35, d 54, and 6 months, whereas no expression was detected on d 4 and d 9. Same expression pattern of Tesmin was confirmed by RT-PCR analysis. Conclusion Tesmin was a time-dependent expression gene in the development of mouse testis, and its expression trend was identical with postnatal mouse spermatogenesis, which sugggested that Tesmin might be involved in mouse spermatogenesis.