自体纤维蛋白膜的制备及其生物物理性能测定

Construction of autoallergic hemaleucin membrane and assessment of its biophysical performance

目的制备自体纤维蛋白膜并测定其物理性能如厚度、抗张强度、伸长率，研究其生物相容性和降解性，以作为载体用于防治腹膜粘连。方法用不同浓度的凝血酶和血浆制备纤维蛋白膜，测定膜的平均厚度、抗张程度及伸长率。采用分组法测定自体纤维蛋白膜植入大鼠体内3d、7d、10d、20d、40d后的生物相容性和降解性。结果采用1mL：10IU凝血酶制备的纤维蛋白膜物理性能最好，组织相容性好，植入大鼠体内1周后可完全降解。结论用凝血酶和血浆制备的纤维蛋白膜具有较好的生物相容性和降解性，有可能作为载体用于减轻腹膜粘连。

Objective In order to find a carrier for mesothelial cell to prevent traumatic peritoneal adhesion, autoallergic hemaleuein membranes were constructed and its biophysical features were checked. Methods The autoallergic hemaleucin membranes were yielded from hematoplasma containing different concentration of thrombase. After measure the mean thickness, level of tensile and extensibility, the hemaleucin membranes were implanted into the rats＇ body, which divided into different groups, to observe its biocompatibility and degradability after 1 days, 3 days, 7 days, 10 days, 20 days postoperatively. Results The autoallergic hemaleucin membranes constructed from hematoplasma containing 10 IU thrombase per 1 mL had the best biophysical performance and best biocompatibihty and degradability, which fully degraded in rat＇s body in 1 week. Conclusions The hemaleucin membrane constructed from hematoplasma and thrombase has good bioeompatibility and degradability which can be used as a carrier for the prevention of traumatic peritoneal adhesion.