犬脊柱放疗对脊髓血管形态及血流量的影响

A study of the ultrastructure of the blood vessel and the blood blow in beagle spinal cord after radiotherapy

目的:观察成年比格犬脊柱放射治疗对脊髓血管形态及血流量的影响。方法:将20只比格犬以随机数字表法随机分为二组,空白对照组4只,放射治疗组16只。在10～14d内,对放射治疗组比格犬胸9～10椎体给予平均总剂量为60Gy的放疗。放射治疗结束3个月后,采用放射性生物微球技术对二组犬胸9～10节段相同部位、相同位置脊髓血流量进行测定。取二组犬胸9～10节段相同部位、相同位置脊髓,用电镜观察二组犬血管超微结构变化;血管免疫组化染色后,测量脊髓血管平均管径和平均截面积。结果:放射治疗组的脊髓组织的血管平均直径、平均截面积和血流量显著低于空白对照组,差异有统计学意义(P<0.05)。透射电镜观察显示放射治疗组的脊髓血管形态及内皮细胞超微结构同空白对照组相比均有明显的病理改变。结论:放射治疗可影响脊髓血管形态及血流量,这可能是放疗所致脊髓功能障碍的重要原因之一。

Objective： To observe the ultrastructure of the blood vessel and the blood blow in beagle spinal cord after radiotherapy. Methods： Twenty purebred beagles were divided into 2 groups randomly. Mimic a beagle clinical model of tumor in the ninth and tenth thoracic vertebrae. Then the second group beagles were irradiated by the radiotherapy,the total irradiation doses were 60 Gy in 10414 d. Measured the blood flow in the spinal cord injury by the radiolabelling microsphere technique on third month after radiation. The samples of spinal cord were taken out from the same locum of the ninth and tenth thoracic vertebrae. All the samples were observed by the electron microscope and evaluated by vessel staining assay. Results： The results showed that the average diameter, average sectional area and the blood blow of the radiothera- py group were all lower than the blank group. The configuration and ultrastructure of vessels were also different between two groups. sel and the blood blow in bea process of spinal cord injury in Conclusion： The radiotherapy can affect the ultrastructure of the blood vesgle spinal cord, which plays an important role in the pathophysiological duced by the radiotherapy.