摘要
本文研究青钱柳多糖(polysaccharides isolated from Cyclocarya paliurus(Batal.)Iljinskjk,PCP)对人胃癌MGC_803细胞生长的影响。采用噻唑蓝(MTT)法检测PCP对MGC_803细胞生长的影响;Annexin法检测PCP对MGC803细胞诱导凋亡的作用。实验结果表明PCP在50、100、200、400μg/mL浓度条件下,均可极显著性抑制人胃癌MGC_803细胞生长(P<0.01),抑制率可达65.07%,细胞凋亡率可达25.44%。说明青钱柳多糖具有抑制人胃癌MGC803细胞生长的生物活性。
The study was designed to investigate the regular effects of polysaccharides isolated from Cyclocarya paliurus （ Batal. ） Iljinskjk （PCP） on human gastric cancer cell line MGC_ 803 in vitro. MTTassay was applied to detect the proliferation activity of MGC_803 cells which were treated by PCP;the apoptosis inducing effects of PCP to MGC 803 cells were treated by Annexin assay. In this study,PCP can inhibit the proliferation of MGC_803 extremely （P 〈 0.01 ） at the concentration of 50,100,200,400 μg/mL. The biggest inhibition rate was up to 65.07% and the apoptosis rate was 25. 44%. Polysaccharide from Cyclocarya paliurus（ Batal. ） Iljinskjk has antitumor bioactivities on human gastric cancer cell line MGC _803.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第6期952-955,共4页
Natural Product Research and Development
基金
教育部长江学者和创新团队发展计划(IRT0540)
食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200806)
关键词
青钱柳多糖
人胃癌MGC_803细胞
MTT
细胞凋亡
Cyclocarya paliurus （ Batal. ） Iljinskjk polysaccharides
MGC_803 cells
MTT
cell apoptosis
