摘要
经DEAE-SepharoseCL-6B、SepharoseCL-6B和Sephadex G-200柱层析,得到一个均一的金丝小栆多糖组分(ZP3c)。ZP3c的中性单糖是由鼠李糖、阿拉伯糖和半乳糖组成,其摩尔比为1:2.13:19.3。经红外光谱测定,ZP3c的酯化度为41.85%。
a water-soluble polysaccharide (ZP3c) was purified by DEAE-SepharoseCL-6B, Sepharose CL-6B and Sephadex G-200 column chromatography. The purity of ZP3c was verified by high-performance liquid chromatography (HPLC) , specific rotatory power test and agarose gel eleetrophoresis. The average molecular weight was estimated to be 1.6 ×10^5 Da. GC analysis result showed that ZP3c was composed mainly of rhamnose, arabinose and galactose in a molar ratio of 1:2.13:19.3. The degree of esterification (DE) value of ZP3c was 41.85% by Fourier transform infrared spectrophotometer.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第6期984-987,共4页
Natural Product Research and Development
基金
江南大学食品学院青年博士科研创新开放基金(FS-200801)
关键词
金丝小栆
多糖
分离纯化
组成
Zizyphus jujuba cv. Jinsixiaozao
polysaccharide
isolation and purification
composition