姜黄素对溃疡性结肠炎大鼠肠黏膜内PPARγ、NF—κB和TNF—α表达的影响

Effects of Curcumin on Expression of PPARγ, NF - κB and TNF - α in Intestinal Mucosa of Rat Model with Ulcerative Colitis

目的观察姜黄素对大鼠溃疡性结肠炎的抗炎疗效，探讨其可能机制。方法雄性健康SD大鼠60只，随机分4组（每组15只）：A组，正常对照组；B组，模型组；C组，姜黄素治疗组；D组，地塞米松治疗组。造模治疗后，行大体损伤评分，HE染色后组织损伤评分，用免疫组化S-P法检测组织标本中PPARγ、NF-κB、TNF—α表达并评分。结果大鼠结肠组织大体损伤形态、HE染色评分均显示C、D组分别与B组比较差异有极显著性（P〈0．01），C、D组比较差异有显著性（P〈0．05）。NF-κB、TNF—α在B组表达明显强于C、D组（P〈0．01），且D组表达强于C组。PPARγ在C、D组间表达强于B组，且C组表达强于D组。NF-κB与TNF-α表达呈显著正相关，与PPARγ表达成负相关。结论姜黄素对溃疡性结肠炎大鼠模型有明显疗效，且优于地塞米松。姜黄素可能通过激活PPARγ的表达，抑制NF-κB、TNF—α的表达获得抗炎的效果。

Objective To investigate the effect of curcumin on rat with ulcerative colitis and study the mechanisms of curcumin effect. Methods 60 male Sprague-Dawley rats were randomly divided into 4 groups. Group A： the normal group, Group B： the model group, Group C： Curcumin group, Group D： Dexamethasone group. We graded the changes of histopathologic and macroscopical, graded the expres- sion of PPARγ, NF-κB, TNF-α. Results Curcumin could significantly reduce colon histopathologic and macroscopical injury. Its curative effect was superior to Dexamethasone. Curcumin decreased the expression of NF-κB, TNF-α and improved the expression of PPARγ. Conclusion Curcumin is an effective therapy on TNBs-induced colitis in rats. The curative effect of curcumin is superior to Dexamethasone. Curcumin may reduce colonic inflammation by decreasing the expression of NF-κB, TNF-α and improving the expression of PPARγ.