隐睾睾丸生精功能的损害

The Apoptosis of Reproductive Cell Damage to the Spermatogenesis Function in Cryptorchidism Testicle

目的研究大鼠隐睾睾丸组织生精功能，了解睾丸生殖细胞凋亡。方法建立隐睾大鼠动物模型，获得单侧隐睾大鼠（A组）12只，双侧隐睾大鼠（B组）10只，空白对照正常大鼠（C组）10只。当日龄为60d时，切取大鼠双侧睾丸组织，行HE染色、Johnsen评分；采用原位缺口末端标记法检测生殖细胞凋亡。结果A、B组隐睾睾丸组织Johnsen评分较对照组显著降低（P〈0．05），生精功能明显破坏，生殖细胞凋亡指数显著增加（P〈0．05）。结论大鼠隐睾睾丸组织生精功能受到损害，生殖细胞凋亡增加。

Objective To study the impairment of spermatogenesis function and the apoptosis of reproductive cell in rat crytorchidism testicle. Methods Thirty two male Sprague Dawley rats were divided into 3 groups：Group A （n = 12）,unilateral cryptorchidism; Group B （n = 10）,bilateral cryptorchidism, and Group C （n = 10） ,control group. Group A and B obtained from the cryptorchidism model was induced by 17-β estradiol. Estimation of spermatogenesis by application of the Johnsen score revealed impairment of germinal cell maturation at day 60 postpartum. Germ cell apoptosis was detected by TdT-mediated dUTP-X nick end labeling in three groups. Result Compared with group C, the impairment of spermatoge-nesis function of testicular tissue of each cryptorchidism in group A and B were obviously （ P 〈 0. 05 ） , Germ cell apoptosis index increased significantly （P 〈 0. 05 ）. Conclusion The spermatogen- esis function of cryptorchidism testicular tissue in rats was damage, the increase of reproductive cell apoptosis associated with the damage.