RNA干扰及其在口蹄疫病毒防御中的应用
被引量:1
摘要
RNA干扰(RNA interference,RNAi)是一种真核生物细胞在转录后引发基因沉默的分子机制。目前,RNAi的分子机制及其功能仍然有待更加细致地阐明,但作为一种反向遗传学手段已经在基因功能研究中广泛运用,并已作为一种治疗策略运用于人类抵抗重大遗传性疾病和病毒性疾病的研究当中。本研究将对RNAi的分子机制及其在抗口蹄疫病毒功能方面的研究作出简要的概括和展望。
出处
《江苏农业科学》
CSCD
北大核心
2009年第6期10-13,共4页
Jiangsu Agricultural Sciences
基金
甘肃省科技重大专项计划项目(编号:0801NKDA034)
参考文献29
-
1岳枫,马文丽,郑文岭.RNAi的不同沉默机制及其应用[J].生物技术通报,2004,20(3):5-7. 被引量:4
-
2Kim D H, Behlke M A, Rose S D, et al. Synthetic dsRNA dicer substrates enhance RNAi potency and efficacy [ J ]. Nat Biotechnol, 2005,23:222 - 226.
-
3Sontheimer E J. Assembly and function of RNA silencing complexes [J]. Nat Rev Mol Cell Biol,2005,6:127 -138.
-
4Xie Z,Johansen L K,Gustafson A M ,et al. Genetic and functional diversification of small RNA pathways in plants [ J ]. Plos Biol, 2004, 2 :E104.
-
5Svoboda P,Anger M ,Bernstein E,et al. RNAi and expression of retrotransposons MuERV - L and IAP in preimplantation mouse embryos [ J]. Dev Biol,2004,269:276 - 285.
-
6Voinnet O. Induction and suppression of RNA silencing:insights from veral infection[ J]. Nat Rev Genet,2005 ,6 :206 - 220.
-
7Schutz S, Sarnow P. Interaction of viruses with the mammalian RNA interference pathway[ J]. Virology,2006,344( 1 ) :151 - 157.
-
8Lecellier C H, Dunoyer P, Arar K, et al. A cellular microRNA mediates antiviral defense in human ceils [ J ]. Science, 2005,308 : 557 - 560.
-
9Chen W. RNA silencing: a remarkable parallel to protein -based immune systems in vertebrates [ J ]. FEBS Lett, 2005,579 : 2267 - 2272.
-
10Weiler K S, Wakimoto B T. Heterochromatin and gene expression in drosophila[ J]. Annu Rev Genetics, 1995,29:577 - 605.
二级参考文献20
-
1Gregory J,Hannon. Nature, 2002, 418: 244-251.
-
2Marjori Matzke, et al. Science, 2001, 293: 1080-1083.
-
3SaydaIII Elbashir,et al. Genes, 2001,15: 188-200.
-
4Nathaniel R. Dudley, et al. PANS, 2002, 99: 4191-4196.
-
5Paul Ahlquist. Science, 2002, 296: 1270-1273.
-
6Alla Grishok, et al. Science, 2000, 287: 2494-2497.
-
7Graham T,cheatham, et al. Science,1999, 286: 2305-2309.
-
8Hong wei li ,et al. Science, 2002, 296: 1319-1321.
-
9Innokenti,Toulokhonov, et al. Science, 2001, 292: 730-733.
-
10Vicki Vance, et al. Science, 2001, 292: 2277-2280.
共引文献3
-
1邓小莉.RNA干涉技术[J].平原大学学报,2005,22(3):104-109. 被引量:1
-
2陶彦彬,蒋建雄,易自力,李骏智.功能基因组学及其研究方法[J].生物技术通报,2007,23(5):61-64. 被引量:9
-
3王卉,郑澜,林丽容,奉悦,张民,倪柳.Imp-L3表达量降低与规律运动对老龄果蝇心脏功能的影响[J].西安体育学院学报,2016,33(3):335-342.
同被引文献21
-
1程小星,蹇锐,邓少丽,蒋静.RNA干扰文库及其在功能基因组学研究中的应用[J].生物化学与生物物理进展,2005,32(3):195-198. 被引量:2
-
2李婧,付汉江,朱捷,邢瑞云,凌世淦,孙志贤,郑晓飞.利用双向启动子型RNA干涉载体构建随机RNA干涉文库的初步研究[J].军事医学科学院院刊,2007,31(3):204-207. 被引量:1
-
3HANNON G J. RNA interference [ J]. Nature,2002,418:244-251.
-
4DENLI A M, HANNON G J. RNAi: an ever-growing puzzle[J]. Trends Biochem Sci,2003,28 : 196-201.
-
5HAMMOND S M, CAUDY A A, HANNON G J. Post-transcriptional gene silencing by double-stranded RNA[ J]. Nat Rev Genet,2001,2 : 110-119.
-
6FIRE A, XU S, MONTGOMERY M K, et al. Potent and specific genetic interferenxe by double-stranded RNA in Caenorhabditis elegans [ J ]. Nature, 1998,391 : 806-811.
-
7HAMILTON A J, BAULCOMBE D C. A species of small antisense RNA in posttranseriptional gene silencing in plants [ J ]. Science, 1999,286:950-952.
-
8KETTING R F, PLASTERK R H A. A genetic link between cosuppression and RNA interference in C. elegans [ J ]. Nature, 2000,404:296-298.
-
9NAPOLI C, LEMIEUX C, JORGENSEN R A. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans [ J ]. Plant Cell, 1990, 2 : 279-289.
-
10CHUANG C F, MEYEROWITZ E M. Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana [J]. Proc Natl Acad Sci,USA,2000,97:4985-4990.
-
1韩美清,赵致.转录后基因沉默及其在植物中的应用[J].生命的化学,2003,23(3):229-231.
-
2ZHU Hui,GUO HuiShan.The role of virus-derived small interfering RNAs in RNA silencing in plants[J].Science China(Life Sciences),2012,55(2):119-125. 被引量:5
-
3JIANG Lin,WEI ChunHong,LI Yi.Viral suppression of RNA silencing[J].Science China(Life Sciences),2012,55(2):109-118. 被引量:8
-
4科学出版社新书[J].中国生物工程杂志,2009,29(2):124-124.
-
5石林丹.拟南芥中Importin β蛋白负向调节MicroRNA活性[J].农业生物技术学报,2011,19(6).
-
6陈晓彤,王允山,吉爱国.MiRNA在特发性肺纤维化中的作用[J].生命的化学,2012,32(5):419-422. 被引量:1