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地衣芽孢杆菌1411-1羽毛角蛋白固体发酵工艺的优化及角蛋白酶的初步研究 被引量：4

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摘要 对角蛋白高效降解菌地衣芽孢杆菌菌株1411-1固体发酵条件进行了优化,着重考察了培养基含水量、起始pH值、温度、接种量及外源添加物的影响,并确定了优化的发酵周期,同时对角蛋白酶的提取进行了初步研究。研究结果表明,1411-1的最适固体发酵条件为:培养基含水量75%,pH值10.0,温度40℃,菌液接种量0.15ml/g,发酵周期88h,氮源对固体发酵有显著的抑制作用,碳源的影响不显著;角蛋白酶的最适提取条件为:以碳酸钠-碳酸氢钠缓冲液(pH值10.0)作为提取剂,4℃下提取60min。
作者 李小会 何斌 李雪影 王玲 史玉峰 王睿勇
机构地区 南京大学生命科学学院
出处 《江苏农业科学》 CSCD 北大核心 2009年第6期334-336,共3页 Jiangsu Agricultural Sciences
基金 国家自然科学基金(编号:30571354) 江苏省科技支撑计划(编号:BE2008405)
关键词 羽毛角蛋白 地衣芽孢杆菌 固体发酵 条件优化 角蛋白酶
分类号 S816.3 [农业科学—饲料科学]
  • 相关文献

参考文献11

  • 1Arai K M, Takahashi R, Yokote Y, et al. Amino acid sequence of feather keratin from fowl [ J ]. European Journal of Biochemistry, 1983,132:501 - 507.
  • 2McGovem V. Recycling poultry feathers:more bang for the cluck [ J]. Environmental Health Perspectives,2000,108 ( 8 ) :366 - 369.
  • 3Ionata E, Canganella F, Bianconi G, et al. A novel keratinase from Clostridium sporogenes by. pennavorans by. nov. , a thermotolerant organism isolated from solfataric muds [ J ]. Microbiological Research,2008,163:105 -112.
  • 4Suntornsuk W, Suntornsuk L. Feather degradation by Bacillus sp. FK46 in submerged cultivation [ J ]. Bioresouree Technology, 2003, 86,239 - 243.
  • 5Singh C J. Exocellular protease of Malbranchea gypsea and their role in keratin deterioration [ J ]. Mycopathologia, 1999,143 : 147 - 150.
  • 6Marcondes R N,Taira C L,Vandresen D C,et al. New feather- degrading filamentous fungi [ J]. Microbial Ecology ,2008,56:13 - 17.
  • 7Gushterova A, Vasileva - Tonkoval E, Dimova E, et al. Keratinase production by newly isolated Antarctic actinomycete strains [ J ]. World Journal of Microbiology and Biotechnology, 2005,21 : 831 - 834.
  • 8张玲,朱晓飞,汤睿,赵平芝,王睿勇.耐热链霉菌B221产生角蛋白酶的液体发酵条件研究[J].江苏农业科学,2007,35(4):187-189. 被引量：7
  • 9董荣斌,张玲,朱晓飞,赵平芝,王睿勇.耐热链霉菌B221降解羽毛角蛋白的固体发酵条件研究[J].江苏农业科学,2007,35(6):249-251. 被引量：11
  • 10Lin X, Lee C G, Casale E S, et al. Purification and characterization of a keratinase from a feather degrading Bacillus licheniformis strain [ J]. Applied and Environmental Microbiology, 1992,58 : 3271 - 3275.

二级参考文献21

  • 1McGovern V.Recycling poultry feathers:more bang for the cluck[J].Environmental Health Perspectives,2000,108 (8):366-369.
  • 2Anbu P,Hilda A.Purification of keratinase from poultry farm isolate Scopulariopsis brevicaulis and statistical optimization of enzyme activity[J].Enzyme and Microbiological Technology,2005,36:639 -647.
  • 3Wang X,Parsons C M.Effect of processing systems on protein quality of feather meal and hair meals[J].Poultry Sciences,1997,76:491-496.
  • 4Letourneau F,Soussotte V,Bressollier P,et al.Keratinolytic activity of Streptomyces sp.S.K1 -02:a new isolated strain[J].Letters in Applied Microbiology,1998,26:77-80.
  • 5Bockle B,Galunsky B,Muller R.Characterization of a keratinolytic serine protease from Streptomyces pactum DSM 40530[J].Applied and Environmental Microbiology,1995,61:3705-3710.
  • 6Lin X,Lee C G,Casale E S,et al.Purification and characterization of a keratinase from a feather degrading Bacillus licheniformis strain[J].Applied and Environmental Microbiology,1992,58:3271 -3275.
  • 7Nickson W J.Keratinase[J].Biochem Biophys Acta,1963,77:87-99.
  • 8Suntornsuk W,Suntornsuk L.Feather degradation by Bacillus sp.FK 46 in submerged cultivation[J].Bioresource Technology,2003,86:239-243.
  • 9Rain G,Priya R.Microbial keratinase and their prospective application:an overview[J].Appl Microbiol Biotechnol,2006,70:21-33.
  • 10Friedrich A B,Antranikian G.Keratin degradation by Fervidobacterium pennavorans,a novel thermophilic anaerobic species of the order thermotogales[J].Applied and Environmental Microbiology,1996,62:2875-2882.

共引文献15

同被引文献40

引证文献4

二级引证文献8

江苏农业科学
2009年 第6期

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