川崎病患儿血清中hs-CRP与基质金属蛋白酶及抑制剂联合检测的临床意义

Roles of matrix metalloproteinases,tissue inhibitor of matrix metalloproteinase and hs-CRP in Kawasaki disease

目的检测基质金属蛋白酶(MMP)-2、MMP-9、基质金属蛋白酶抑制剂(TIMP)-1及hs-CRP在川崎病患儿血清中的表达,并探讨其与冠状动脉损伤的关系。方法选取151例川崎病患儿作为观察组(无冠状动脉损伤40例,有冠状动脉损伤111例),60例健康儿童作为对照组,采用酶联免疫吸附实验(ELISA)检测血清中MMP-2、MMP-9和TIMP-1含量,应用终点散射比浊法检测血清hs-CRP的含量。结果MMP-2,MMP-9及hs-CRP含量在冠脉损伤组及无冠脉损伤组与对照组比较差异均有显著性,在冠脉损伤组量最高(P<0.05);川崎病患儿中MMP-2、MMP-9及TIMP-1之间的检测量呈正相关(P<0.05)。结论MMP-2、MMP-9、TIMP-1及hs-CRP在川崎病的发生发展中可能起重要作用,MMP-2、MMP-9及hs-CRP的联合检测可能对判断病变程度有重要帮助。

Objective To examine the expression of matrix metalloproteinases （MMP）-2, -9, tissue inhibitor of matrix metalloproteinase （TIMP）-I and hs-CRP, and their relationship with coronary artery in children with Kawasaki disease. Methods One hundred and fifty-one children with Kawasaki disease （ 111 cases with coronary artery damage and 40 cases without） and 60 healthy children were enrolled. The expression of MMP-2, MMP-9 and TIMP-1 was detected using ELISA, and the hs-CRP concentration was measured using the endpoint nephelometry. Results There were significant differences in the level of MMP-2, MMP-9 and hs-CRP between the patients with or without coronary artery damage and the healthy children （ P 〈 0.05 ）. The levels of MMP-2, MMP-9 and hs-CRP were the highest in the cardiovascular damage group （P 〈 0.05）. There were positive correlations between MMP-2, MMP-9 and TIMP-1 in children with Kawasaki disease. Conclusions MMP-2, MMP-9, TIMP-1 and hs-CRP may play important roles in the development of Kawasaki disease. The combined measurement of MMP-2, MMP-9 and hs-CRP may be useful in the evaluation of the severity in children with Kawasaki disease.