期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

大鼠坐骨神经结扎后背根神经节神经生长因子的表达变化

Changes of NGF expression in dorsal root ganglia after sciatic nerve ligation in rat
下载PDF
导出
摘要 目的：观察坐骨神经结扎后神经生长因子（NGF）在背根神经节的表达变化。方法：健康SD大鼠随机分为正常对照组、假手术对照组和坐骨神经结扎组，实验组结扎后分别存活1、3、5、7、14、21、28d，取腰4～6背根神经节（DRG），行NGF免疫组织化学显色结合图像分析技术分析其表达变化。结果：结扎后1d DRG NGF表达无明显变化；3d开始下降，7d达最低值，持续到21d；28d恢复正常。结论：神经结扎后DRG神经元NGF表达变化可能参与了神经损伤后的可塑性。 Objective： To study the expression changes of nerve growth factor （NGF） in dorsal root ganglion （DRG） after sciatic nerve ligation in rats. Methods： Totally 45 healthy adult Sprague-Dawley rats were randomly divided into a normal group, a sham-operation group and an experimental group, and respectively survived for 1, 3, 5, 7, 14, 21 and 28 d after sciatic nerve ligation （at each time point）. The distribution and changes of NGF in L46 DRG were detected by irnmuno-staining. Then the gray scale values of NGF-immunopositive products were analyzed. Results： No change in NGF expression was found in DRG neurons after sciatic nerve ligation at 1 d. However, it began to drop at 3 d, then peaked at 7 d and lasted to 21 d after ligation. At 28 d after ligation, the expression of NGF returned to normal in DRG neurons. Conclusion： Present results suggest that expression changes of NGF in DRG neurons may be involved in the neuronal plasticity after nerve injury.
作者 郑林丰 吴贤群 许愿忠 张贵祥 王岐本 张建伟 易西南
机构地区 上海交通大学附属第一人民医院放射科 海南医学院解剖学教研室 中南大学湘雅医院 中南大学解剖学与神经生物学系
出处 《解剖学杂志》 CAS CSCD 北大核心 2009年第6期790-792,共3页 Chinese Journal of Anatomy
基金 国家自然科学基金（30760259,30860290） 上海市科委重点项目（07jc14032）.
关键词 坐骨神经结扎 神经生长因子 背根神经节 大鼠 sciatic nerve ligation nerve growth factor dorsal root ganglion rat
分类号 R651.3 [医药卫生—外科学] R338 [医药卫生—人体生理学]
  • 相关文献

参考文献10

  • 1Zheng L F, Wang R, Xu Y Z, et al. Calcitonin gene-related pep tide dynamics in rat dorsal root ganglia and spinal cord following different sciatic nerve injuries [J]. Brain Res, 2008, 1187 (1):20-32.
  • 2Martinez-Lavin M, Solano C. Dorsal root ganglia, sodium chan nels, and fibromyalgia sympathetic pain [J]. Med Hypotheses, 2009,72(1) :64- 66.
  • 3Shen H, Chung J M, Chung K. Expression of neurotrophin mRNAs in the dorsal root ganglion after spinal nerve injury [J]. Brain Res Mol Brain Res, 1999,64(2) : 186-192.
  • 4Petruska J C, Mendell L M. The many functions of nerve growth factor: multiple actions on nociceptors [J].Neurosci Lett, 2004, 361(1-3):168- 171.
  • 5Sato C, Sakai A, Ikeda Y, et al. The prolonged analgesic effect of epidural ropivacaine in a rat model of neuropathic pain [J]. Anesth Analg, 2008,106 (1) : 313-320.
  • 6Lee S E, Shen H, Taglialatela G, et al. Expression of nerve growth factor in the dorsal root ganglion after peripheral nerve injury [J]. Brain Res, 1998,796(1-2) :99-106.
  • 7臧晓方,张建伟,易西南,张建一,许愿忠,李昌琪.外源性NGF对大鼠坐骨神经切断缝合术后脊髓和背根节CGRP表达的影响[J].神经解剖学杂志,2005,21(5):516-520. 被引量：6
  • 8Li L, Deng Y S, Zhou X F. Downregulation of TrkA expression in primary sensory neurons after unilateral lumbar spinal nerve transection and some rescuing effects of nerve growth factor infusion[J]. Neurosci Res, 2000,38(2): 183-191.
  • 9Ikegami R. Changes of nerve growth factor (NGF) content in-injured peripheral nerve during regeneration: local synthesis of NGF: by Schwann cells [J]. Nippon Seikeigeka Gakkai Zasshi, 1990,64 (7) : 612-622.
  • 10Fukuoka T, Kondo E, Dai Y, et al. Brain-derived neurotrophic factor increases in the uninjured dorsal root ganglion neurons in selective spinal nerve ligation model[J]. J Neurosci, 2001,21 (13):4891-4900.

二级参考文献11

  • 1Van der Kloot W, Benjamin WB, Balezine D. Calcitonin gene-related peptide acts presynaptically to increase quantal size and output at frog neuromuscular junctions. J Physiol, 1998;507 (Pt 3 ) :689 - 695.
  • 2Lee WT, Lee JE, Yang CH et al. Effects of capsaicin on the calcitonin gene-related peptide (CGRP) in the dorsal hom of the rat.Exp Neurobiol, 1992 ; 1 : 13 - 20.
  • 3Changeux J. Molecular biology of the acetylcholine receptor: functional architecture and development regulation. Eur J Neurosci,1991:4:1 - 10.
  • 4Piehl F, Hammarberg H, Tabar Get al. Changes in the mRNA expression pattern, with special reference to calcitonin gene-related peptide, after axonal iujuries in rat motoneurons depends on age and type of injury. Exp Brain Res, 1995 ; 119 : 191 - 204.
  • 5Hall AK, Ai X, Hiekman GE et al. The generation of neuronal heterogeneity in a rat sensory ganglion. J Neurosci, 1997;17:2775 -2784.
  • 6Ai XB, Macphedran SE, Hall AK. Depolarization stimulates initial calcitonin gene-related peptide expression by embryonic sensory neurons in vitro. J Neurosci, 1998 ; 18:9294 - 9302.
  • 7Bulloch K, Milner TA, Prasad A et al. luduction of calcitonin gene-related peptide-like immunoreactivity in hippocampal neurons following ischemia: a putative regional modulator of CNS injury/immune response. Exp Neurol, 1998 ; 15 : 195 - 205.
  • 8Wang S, Yang D, Lippman ME. Targeting Bcl-2 and Bcl-xl with nonpeptide small molecule antagonists. Semin Oncol, 2003 ; 30 :133 - 142.
  • 9Nasu F. Analysis of calcitonin gene-related peptide containing nerve fibres in rat spinal cord using light and electron microscopy.J Electron Microsc, 1999 ;98:267 - 275.
  • 10Auand P. Rudge P, Mathias CJ et al, New autonomic and sensory neuropathy with loss of adrenergic sympathetic function and sensory neuropeptides, Lancet, 1991 ;337 : 1253 - 1254.

共引文献5

解剖学杂志
2009年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部