猫脊髓胆囊收缩素可塑性终末在腰骶背根节全切后板层Ⅱ的变化

Changes of cholecystokinin terminals in lamina Ⅱ of cat spinal cord following dorsal rhizotomy

目的：探讨猫单侧腰骶背根节全切后胆囊收缩素（CCK）可塑性终末的改变。方法：成年雄性猫，分为急性组和慢性组，动物深麻，经灌注固定后取脊髓腰6节段做CCK光镜和电镜免疫组织化学分析。结果：CCK在对照侧主要分布于背角Ⅱ板层，急性组与慢性组手术侧背角CCK免疫反应均明显弱于对照侧，其中以Ⅱ板层最为突出；慢性组手术侧CCK免疫反应强度下降的幅度小于急性组。结论：电镜下观察到的CCK终末可能是光镜下CCK在慢性组回升的结构基础。

Objective： To investigate the changes of CCK terminals in lamina Ⅱ of cat spinal cord following dorsal rhizotomy. Methods： 10 male adult cats were divided into 2 groups： acute group, which survived 7-24 days; chronic group, which survived 2 months. After anesthesia, all cats were perfused, and the L6 segment of the spinal cord was removed and prepared for light and electron microscopy of CCK immunohistochemistry. Results ： CCK immunoreactivity was shown in lamina Ⅱ of dorsal horn in the control side, which was more intensive than that of operation side in both acute and chronic groups. In the chronic group, the intensity of CCK immunoreactivity of operation side decreased slightly compared to that of the acute group. Conclusion： An increase in CCK immunoreactivity shown at the light microscopy level relate to the increased number of CCK-eontaining terminals in lamina Ⅱ under the electronic microscope.