当归补血汤对气虚血瘀大鼠免疫功能及相关基因的调控

Effect of Danggui Buxue Tang(当归补血汤) on immune function and related gene expression in rats with Qi deficiency and blood stasis syndrome(气虚血瘀)

目的:初步探讨当归补血汤益气活血功效的免疫学分子基础。方法:动态观测高(24g/kg)、中(12g/kg)、低(6g/kg)剂量当归补血汤对气虚血瘀大鼠T细胞亚型计数、T淋巴细胞转化、肾上腺重量的作用,并用Real tim e PCR检测当归补血汤对气虚血瘀大鼠IL-1β、TNF-α、NF-κB、MAPK mRNA表达的影响。结果:当归补血汤使气虚血瘀大鼠T淋巴细胞亚群比率由低下逐渐恢复至正常水平,T淋巴细胞转化增殖的能力显著提高;显著降低TNF-α、IL-1β、NF-κB、p38和JNK mRNA的表达。结论:当归补血汤通过抑制NF-κB、p38MAPK和JNK多条免疫应答信号转导通路,进而调节IL-1β、TNF-α等细胞因子的表达来防治气虚血瘀证。

Objective：To explore the molecular basis of Danggui Buxue Tang（Astragalus and Angelica compatibility（AAC）） on tonifying Qi and controlling blood circulation.Methods： Effects of AAC on T-lymphocyte transformation,T cell subtypes count and adrenal gland weight were determined at dosage of high（24g/kg）,medium（12g/kg） and low（6g/kg） as well as mRNA expressions of IL-1β,TNF-α,NF-κB,p38MAPK and JNK in blood cells of rats with Qi deficiency and blood stasis syndrome were measured with real-time fluorescent quantitative-PCR at dosage of high（24g/kg） at 5th,14th and 28th day.Results： Compared with syndrome model,high dose AAC（24g/kg） could obviously improve T-lymphocyte subtype counts,T-lymphocyte transformation,modulate PHA axis and decrease mRNA expression of IL-1β,TNF-α,NF-κB,p38MAPK and JNK.Conclusion： The effects of AAC on Qi deficiency and blood stasis syndrome was brought from the regulating of cytokine network,NF-κB,p38MAPK and JNK signal transduction pathways included.