摘要
目的:探讨中药成分LLN对大鼠急性心肌损伤的保护作用。方法:用异丙肾上腺素(ISO)30mg/kg两次注射造成大鼠心肌损伤模型,LLN大(400mg/kg)、中(200mg/kg)、小剂量组(100mg/kg)及丹参酚酮(400 mg/kg)灌胃,观察对急性心肌损伤的大鼠心电图△J的变化,计算心脏指数,测定血清CK和LDH,同时测定心肌匀浆中Na+-K+-ATPase、Ca2+-ATPase、Mg2+-ATPase活性。结果:ISO模型组心肌系数和血清CK、LDH显著升高;Na+-K+ATPase、Ca2+-ATPase、Mg2+-ATPase的活性显著下降。与模型组比,LLN各剂量组和丹参组心电图△J降低,心脏系数和血清CK、LDH显著下降,Na+-K+-ATPase、Ca2+-ATPase、Mg2+-ATPase的活性升高。结论:LLN对异丙肾上腺素造成的急性心肌损伤具有保护作用,其保护机制与提高Na+-K+-ATPase、Ca2+-ATPase、Mg2+-ATPase活性,减轻细胞内钙超载有关。
Objective:To study the protection and possible mechanisms of LLN in acute myocardial injury in rats.Methods: Acute myocardial injury induced by hypodermic injecting isoprenaline(ISO).SD rats were divided into six groups at random.They were control group,the ISO group(NS 10ml/kg + ISO 30mg/kg·d),the LLN group(400mg/kg + ISO 30mg/kg·d),the LLN group(200mg/kg + ISO 30mg/kg·d),the LLN group(100mg/kg + ISO 30mg/kg·d) and the Danshen group(400mg/kg + ISO 30mg/kg·d).The effect of LLN in myocardium was observed by△J of ECG and measured by cardiac index.The activities of Na+-K+ATPase,Ca2+-ATPase and Mg2+-ATPase were measured.Results: In the ISO group,compared with the control group,cardiac index and CK,LDH content in serum were higher.The activities of Na+-K+ATPase,Ca2+-ATPase and Mg2+-ATPase were decreased.In the LLN group and the Danshen group,the cardiac index,△J of ECG and CK,LDH content in serum of rats injured by ISO were relieved.The activities of Na+-K+-ATPase,Ca2+-ATPase and Mg2+-ATPase were higher than those in the ISO group.Conclusion: The LLN can alleviate rat myocardium from acute ischemic injury by isoprenaline,one of the mechanisms is to inhibit the overload of Ca2+ in ischemia cardiac muscle cells by increasing the activities of Na+-K+-ATPase,Ca2+-ATPase and Mg2+-ATPase.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2009年第5期22-24,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家重点基础研究发展计划(2007CB512604)