摘要
目的研究长寿命自由基在辐射损伤中的作用。方法将细胞分为对照组、抗坏血酸、氨基胍和人参皂甙处理组,照射剂量分别为2、4、6、8 Gy,实验组照射30 min后加入药品,用MTT方法检测细胞增殖性的变化,用微核率检测细胞DNA的损伤程度。结果12 h和24 h检测各剂量点处,实验组OD值均比对照组显著增加,而微核率却显著减少。结论长寿命自由基在电离辐射诱导细胞的损伤中起着重要作用,而抗坏血酸、氨基胍和人参皂甙可以有效的清除长寿命自由基,减轻辐射损伤。
Objective To investigate the effect of long- lived free radical on cellular damage induced by ionization radiation. Methods Four groups were founded by control, AsA, amide, and ginsenoside, the irradiation doses were 2, 4, 6, and 8 Gy, respectively. MTTwas measured at 12 and 24 hours after ionization radiation and the damage of DNA was measured by micronuclei ratio. Results As compared with the control group, the OD values of operated groups significantly increased, while the micronuclei ratio significantly decreased. Conclusions The long - lived free radical could induce the damage of ionization radiation; AsA, amide, and ginsenoside can efficiently remove them and relieve the damage.
出处
《实用预防医学》
CAS
2010年第1期7-8,共2页
Practical Preventive Medicine
基金
吉林省科技厅白求恩基金资助课题(200705221)
