摘要
目的研究Notch1、P21WAF1/Cip1及活化caspase3在肝细胞癌中表达的相关性。方法利用免疫组织化学检测60例肝细胞癌(HCC)和15例正常肝组织中Notch1、P21WAF1/Cip1及活化caspase3的表达。结果HCC中Notch1、P21WAF1/Cip1及活化caspase3的阳性率和阳性表达强度显著低于正常肝组织(P<0.01);Notch1、P21WAF1/Cip1及活化caspase3的表达强度与HCC分级和肿瘤大小密切相关,肿瘤分化愈差和肿瘤直径愈大,3种蛋白表达愈弱(P<0.01);在HCC中,P21WAF1/Cip1和活化caspase3的表达强度与Notch1表达强度呈显著正相关(r分别为0.76和0.71)。结论提示Notch1信号通路可能是通过影响细胞周期和细胞凋亡在HCC发生发展过程中发挥负性调控作用。
Objective To study the correlation among the expression of Notch1, P21^WAF1/Cip1 , and activated caspase3 in hepatocellular carcinoma. Methods Irnmunohistochemistry was used to detect the expression of Notch1, P21^WAF1/Cip1 , and activated caspase3 in 60 cases of hepatocellular carcinoma （HCC） and 15 cases of normal liver tissue. Results The positive expression rates and intensities of Notch1, P21^WAF1/Cip1 , and activated caspase3 in HCC were significantly lower th.an those in normal liver tissues （P〈0.01）. The expression intensities of Notch1, P21^WAF1/Cip1 and activated caspase3 were closely related to differentiated degree and tumor size in HCC, the poorer differentiation and the larger size of tumor, the weaker expression of 3 kinds of proteins （P 〈 0.01）. The expression intensities of P21^WAF1/Cip1 and activated caspase3 had significant positive correlation with that of Notchl in HOC （r = 0. 76, r = 0.71）. Conclusions The results suggest that the Notchl signaling may exert negative regulation function through affecting cell cycle and apoptosis during genesis and development of HCC.
出处
《实用预防医学》
CAS
2010年第1期44-46,共3页
Practical Preventive Medicine
