健脑颗粒质量标准研究

Study on Quality Standard of Jiannao Granules

目的建立健脑颗粒的质量控制标准。方法采用薄层色谱法对健脑颗粒中川芎、枸杞子进行定性鉴别,采用高效液相色谱法测定淫羊藿中主要成分淫羊藿苷的含量。结果供试品溶液色谱中,在与川芎、枸杞子对照药材溶液色谱相应位置上显相同颜色的斑点;淫羊藿苷进样量在0.20～1.60μg范围内与峰面积呈良好线性关系(r=0.9999),平均回收率为99.36%,RSD=0.80%(n=6)。结论所用方法快速、准确,可用于健脑颗粒的质量控制。

Objective To establish the quality standard of Jiannao Ganules. Methods The identification of Rhizoma chuanxiong and Fructus lycii was carried out by TLC; and the content of icariin, the principal component in Herba epimedii, was determined by HPLC. Results In the chromatography of sample, the same-colored speckles appeared at the corresponding position of the reference substance of Rhizoma chuanxiong and Fructus lycii. The linear range of ieariin was 0. 20 - 1.60 μg（ r =0. 999 9）, and the average recovery rate was 99.36% （ RSD =0. 80% ）. Conclusion The method is quick and accurate, which can be used for the quality control of Jiannao Granules.