不同大小丹蓝微丸中丹皮酚含量比较

Comparision of Paeonol Contents in Danlan Pellets of Different Sizes

目的：用HPLC法测定不同大小丹蓝微丸中丹皮酚的含量。方法：色谱柱为Agilent TC-C18（250mm×4.6mm,5μm）,流动相为甲醇-水（65∶35）,流速为1.000mL·min-1,检测波长为274nm。结果：丹皮酚在0.8128-4.064μg范围内与峰面积积分值呈良好线性关系（R2=0.9998）;加样回收率为97.24%（RSD=1.95%）。结论：该法简便、准确、重复性好,可用于丹蓝微丸中丹皮酚的含量测定,随着微丸直径的减小,丹皮酚的含量有一定增加。

Objective：Determination of paeonol in Danlan Pellets of different sizes byhPLC.Methods：AnhPLC test was performed using Agilent TC-C18（250mm×4.6mm,5μm） as column,methanol-water （65∶35）as mobile phase,with the flow rate set 1.000mL·min-1,detection wavelength at 274nm.Results：The linear range was 0.8128-4.064μg（R2=0.9998）.The average recovery was 97.24%（RSD=1.95%）. Conclusions：This method is rapid,accurate,and reproducible,which can be used to determine the content of paeonol in Danlan Pellets .With sizes of pellets decrease, contents of paeonol increase.