用光散射技术检测牛乳蛋白质时的影响因素研究

Study of Influence Factors of Milk Protein Detection by Light Scattering

虽然利用光散射技术可以实现对牛乳中蛋白质成分的检测,但在检测过程中有很多因素会影响其检测精度。本文在对牛乳中蛋白质的光散射特性进行分析的基础上,应用米氏散射理论和光学仿真软件,研究了检测过程中光源波长、散射角度和环境温度等因素对牛乳中蛋白质散射光强的影响。通过仿真实验,确定最佳的光源波长为1333nm或1600nm,较好的散射角度为0°、±30°和±90°,适宜的检测环境温度为40℃～60℃,从而为提高仪器的检测精度提供了方案与数据上的支持。该方法不需要现成检测仪器的参与,因而可避免检测仪器的繁琐操作,杜绝人为误差。另外,它还可根据要求随意调整实验中各环节的参数,以节约研究成本。

Although the light scattering technology can be used to detect the proteins in milk, there are many factors which have influence on its detection accuracy in practical use. The light scattering characteristics of the proteins in milk are analyzed. On the basis of this analysis, the Mie scattering theory and optical simulation software are used to study the factors such as laser wavelength, scattering angle and ambient temperature ete which have influence on the scattering light intensity of the proteins in milk to be detected. Through simulation, the best laser wavelength is determined to be 1333nm or 1660nm; the better scattering angles are determined to be 0°, ±30° and ±90° and the suitable ambient temperature is from 40℃ to 60 ℃. This can provide the data support for the improvement of the detection accuracy of an instrument. The method does not need any actual detection instruments. Thus, it can avoid the complicated operation of a detection instrument and eliminate any artificial errors. Moreover, all parameters can be adjusted according to the requirement in experiment and the study cost can be saved.