金黄地鼠舌癌发生过程中增殖性细胞核抗原的表达及淋巴管密度和面积与淋巴道转移的相关性

Expression of PCNA,lymphatic vessel density,lymphatic vessel area and their relationship with lymphaticmetastasis in the hamster tongue cancer development

目的通过观察金黄生鼠舌癌发生过程中增殖细胞核抗原(PCNA)的表达情况,分析淋巴管密度和面积的变化与舌癌恶性程度及淋巴道转移的相关性,探讨淋巴管生成与淋巴道转移的关系。方法用1.5%丙烯酰胺-N,N-二甲基-N-丁基-N-甲基丙烯酸乙酯溴化铵(DMBA)丙酮溶液合并创伤方法,诱导金黄地鼠舌癌模型42只,每2周取材7只。采用HE染色确定舌癌的病理变化,PCNA和桥粒斑蛋白(Desmoplakin)免疫组织化学双标记法显示淋巴管增生情况,并测量肿瘤和淋巴管内皮细胞增殖指数(PI)、淋巴管密度(LVD)和淋巴管面积(LVA)作统计学分析。结果金黄地鼠舌癌的形成经历了非典型增生-原位癌-早期浸润癌的过程;在非典型增生组PI(91.55),LVA(7570.23),LVD(2.50);原位癌组PI(113.36),LVA(12105.45),LVD(3.73);早期浸润癌组PI(124.67),LVA(14524.33),LVD(5.33),各组间LVA,LVD,PI差异显著(P<0.05),且PI与LVA及LVD呈正相关(P<0.05);淋巴结转移组PI(130.50),LVA(15430.67),LVD(6.17),非转移组PI(113.00),LVA(12711.67),LVD(3.67),两组差异显著(P<0.05)。结论舌癌发生过程中存在新生淋巴管,舌癌形成过程中微淋巴管的LVA和LVD值增大,与舌癌的恶性程度呈正相关,与淋巴道转移呈正相关。

Objective To investigate the relationship of the lymphangiogenesis and lymphatic metastasis by observing the expression of proliferating cell nuclear antigen in the hamster tongue cancer development and analyzing the correlations of lymph vessel density （LVD）, lymphatic vessel area （LVA） and malignance or lymphatic metastasis of tongue cancer ceils. Methods Forty-two hamster tongue cancer models were induced by painting 1.5% 7, 12-dimethylben （a） anthracene （DMBA） acetone solution after scratching, 7 models were sacrificed every two weeks. The pathological changes of tongue cancer were determined by the HE staining. The lymphatic hyperplasia was determined by PCNA and Desmoplakin（ DP I ＋ I] ） Immunohistochemical double staining. The tumors and lymphatic endothelial cell proliferation index （proliferation index, PI）, lymphatic vessel density （LVD） and lymphatic vessel area （LVA） were measured and statistically analyzed. Results The oncogenesis of tongue was divided into three stages： atypical hyperplasia, carcinoma in situ and early invasive stage; atypical hyperplasia group： PI （91.55）, LVA （7570.23）, LVD （2.50）; carcinoma in situ group： PI （113.36）, LVA （12105.45）, LVD （3.73）; early invasive group： PI （124.67）, LVA （14524.33）, LVD （5.33）. The PI, LVA and LVD were compared among groups above （P 〈 0.05）, and the correlation of PI with LVA and LVD was positive （ P 〈 0.05） ; Lymphatic metastasis group： PI （ 130.50）, LVA （ 15430.67）, LVD （6.17）, non lymphatic metastasis group：PI （113）, LVA （1：2711.67）, LVD （3.67）, the PI, LVA and LVD were compared between the two groups above （ P 〈 0.05 ）. Conclusion With the development of hamster tongue cancer, lymphatic endothelial cell proliferation activity increased, suggesting that the lymphangiogenesis existed in the tongue carcinogenesis process; the values of the LVD and LVA increased in the hamster tongue cancer development, which was positively correlated with the cancer cell malignance and lymphatic metastasis.