一种快速辨别糙皮侧耳和肺形侧耳的方法

A Rapid Method for Distinguishing between Pleurotus ostreatus and Pleurotus pulmonarius

目的:建立一种快速、经济的方法辨别糙皮侧耳(P.ostreatus)和肺形侧耳(P.pulmonarius)。方法:在GenBank下载糙皮侧耳和肺形侧耳的ITS序列,经ClustalX序列比对,利用Primer 3设计特异引物组合1F(5′-GATAGATCTGTGAAGTCGTC-3‘)、1R(5′-TCACAATTGGAAAGAAACC-3′)和2R(5′-TGCGTGCTATTGATGAGTGA-3′),最后经PCR扩增和琼脂糖凝胶电泳检测。结果:5个糙皮侧耳菌株均能得到2条带,分别为342bp和459bp。4个肺形侧耳菌株均能扩增到一条446bp的片段。结论:该组引物适合辨别糙皮侧耳和肺形侧耳。

Objective： To set up a rapid and economic method for distinguishing Pleurotus ostreatus and Pleurotus pulmonarius.Method： The Internal Transcribed Spacer sequences of P.ostreatus and P.pulmonarius were downloaded from GenBank,aligned by ClustalX,and then the specific primers,1F（5′-GATAGATCTGTGAAGTCGTC-3′）,1R（5′-TCACAATTGGAAAGAAACC-3′） and 2R（5′-TGCGTGCTATTGATGAGTGA-3′）, were designed by Primer 3.After the PCR amplification,it was detected by agarose gel electrophoresis.Result： Amplified with this set of primers,there were two bands,342bp and 459bp,in all the five strain of P.ostreatus,and a 446bp band in all the four strain of P.pulmonarius.Conclusion： This set of primers was suit for distinguishing between P.ostreatus and P.pulmonarius.