结肠癌组织中癌症高表达蛋白的表达及其临床意义

Expression and clinical significance of highly expressed protein in cancer in colorectal carcinoma tissues

目的:探讨结肠癌组织中癌症高表达蛋白(Hec1)的表达与肿瘤浸润、转移及预后的关系。方法:采用免疫组化SP法检测20例正常结肠组织、40例癌旁组织及癌组织中Hec1的表达,用蛋白印迹法检测上述组织中Hec1蛋白的表达。结果:在正常结肠组织、癌旁组织及癌组织中,Hec1蛋白的阳性表达率分别为0、20.0%和67.5%,各组织中Hec1蛋白的表达差异均有统计学意义,P<0.05。Hec1蛋白的表达与结肠癌的临床病理分期、侵袭转移显著相关,在发生淋巴道转移及低分化的肿瘤组织中,Hec1蛋白高表达。蛋白印迹法结果表明,在正常结肠组织、癌旁组织及癌组织中,Hec1蛋白的平均表达量的分别为0、0.13±0.02、0.87±0.15,差异有统计学意义,P<0.05。结论:Hec1蛋白的过度表达可能在结肠癌的发生和发展中起重要作用,联合应用Hec1蛋白阳性表达率与其临床病理学分级,有助于正确判断患者的预后。

OBJECTIVE：To investigate the relationship between the expression of highly expressed protein in cancer（Hec1）and the infiltration,metastasis and prognosis of colorectal carcinoma.METHODS：The expression of Hec1 was detected in 40 patients with colorectal cancer by SP mimunohistochemistry and Western blot with 20 normal cases as the control.RESULTS：The expressions of Hecl in normal tissues,adjacent normal tissues and colorectal carcinoma tissues were 0,20.0% and 67.5%,and there was significant difference（P0.05）.And the Hecl protein expression in those tissues were 0,0.13±0.02 and 0.87±0.15（P0.05）.The clinical pathological characteristics of colorectal carcinoma and clinical outcome of the patients were associated with the expression of Hec1.Hec1 was highly expressed in cancer tissues with lymph node metastasis and poor differentiation.Especially,a statistical correlation was found with more advanced clinical stages of colorectal carcinoma.CONCLUSIONS：Hec1 may be associated with the development,infiltration and metastasis of colorectal carcinoma.The combination of Hec1 expression in cancer tissues with clinical staging may faliliate the accurate predication of patients＇ prognosis.