肝癌组织CD24基因表达及其临床意义

Expression of CD24 in hepatocellular carcinoma tissues and its clinical significance

目的:探讨CD24基因在原发性肝细胞癌(HCC)组织中表达的特征及其临床病理意义。方法:应用RT-PCR及免疫组化方法检测CD24基因在42例肝癌组织、相应42例癌旁组织中的表达,分析其与临床病理特征的相关性。结果:RT-PCR显示,CD24mRNA阳性表达与肝细胞癌组织分化程度(P=0.019)及有否肝内转移有关(P=0.031),与肝细胞癌患者性别、HBsAg状态、术前AFP水平、肿瘤是否有包膜、肿瘤大小、癌肿数目无关(P>0.05);Elivision法显示,CD24蛋白阳性表达与肿瘤的组织分化程度(P=0.025)、有无肝内转移密切相关(P=0.008),而与患者性别、HBsAg状态、术前AFP水平、肿瘤是否有包膜、肿瘤大小、癌肿数目无关(P>0.05)。HCC组织中CD24表达较癌旁组织明显升高。结论:HCC组织中CD24基因表达能促进HCC的发生、发展和转移,可作为判断HCC恶性程度、评价预后的指标。

OBJECTIVE：To explore the express features and clinical significance of CD24 in HCC tissues.METHODS：The expression of CD24 was detected by RT-PCR and immunohistochemistry in 42 cases of hepatocellular carcinoma and 42 cases of paraneoplastic cirrhosis tissue,and the dependability between CD24 and the characteristics of clinic pathology was analyzed.RESULTS：RT-PCR showed that the expression of CD24 mRNA was significantly correlated with the histological differentiation（P=0.019）and intrahepatic metastasis（P=0.031）,but not with the sex,level of serum HBsAg,level of preoperative serum α-fetoprotein（AFP）,tumor capsula,tumor size,and number of tumor（P0.05）.The method of Elivision showed that the expression of CD24 was significantly correlated with the histological differentiation（P=0.025）and intrahepatic metastasis（P=0.008）,but not with the sex,level of serum HBsAg,level of preoperative serum α-fetoprotein（AFP）,tumor capsula,tumor size,and number of tumor（P0.05）.The expression of CD24 in HCC was obviously higher than that in paraneoplastic cirrhosis.CONCLUSION：The expression of CD24 in HCC can promote the oncogenesis,progression and metastasis of HCC,and CD24 may be an index of malignancy and prognosis in HCC tissues.