人外周血γδT细胞对裸鼠人肺癌移植瘤治疗作用的研究

Therapeutic effect of human peripheral γδT cells on human lung cancer xenograft in nude mice

目的:探讨人外周血γδT细胞对已建立肺癌裸鼠的免疫治疗作用。方法:用人肺癌细胞株A549接种BALB/c裸鼠皮下,建立肺癌裸鼠模型。从健康人外周血中提取γδT淋巴细胞,体外特异性扩增γδT细胞。将已建立肺癌的裸鼠随机分为两组,对照组用RPMI1640培养液,治疗组用γδT细胞分别注入裸鼠腹腔内,观察种植瘤裸鼠生存时间及肿瘤体积,并同时检测肿瘤细胞c-jun的表达。结果:裸鼠接种5×106肺癌细胞后第14天,在接种部位均有肿瘤形成,直径平均为2 mm。在首次治疗后49 d时,对照组、治疗组平均肿瘤体积分别为(0.97±0.32)和(0.46±0.25)cm3,差异有统计学意义,P<0.05。至75 d时,对照组种植瘤裸鼠全部死亡,治疗组生存天数较对照组差异有统计学意义,P<0.05。结论:人外周血γδT细胞对肺癌裸鼠种植瘤具有显著的抑瘤作用,为肺癌免疫治疗提供了一种新的治疗方法。

OBJECTIVE：To explore the immunotherapy effect of peripheral γδT cells in lung cancer nude mice model.METHODS： Xenografted animal model of human lung cancer was established by innoculating human lung cancer A549 cells into BALB/c nude mice subcutaneously.And then the mice were divided into two groups randomly,γδ T cells and control group.γδT cells or RPMI-1640 were respectively injected into abdominal cavity of mice,Tumor volume and he survival rate of two groups were compared.The expression of c-jun was tested on tumor cell.RESULTS： The nude mice inoculated with 5×106 lung cancer cells,after fourteen days in the inoculation site a diameter of 2-3 mm tumor was formed.In the first 49 days after treatment determined two group tumor average size were（0.97±0.32） cm^3 and（0.46±0.25） cm^3 respectively,the treatment groups with a very significant difference compared with the control group in the tumor size（P〈0.05）.After 75 days,all the mice in control group died,The survival time of treat ment group was longer than that of control group（P〈0.05）.CONCLUSION： Human peripheral γδT cells can inhibit the growth of human lung cancer xenograft in nude mice effectively.