子宫内膜癌组织COX-2和VEGF及KDR蛋白表达临床意义的探讨

Expressions of COX-2,VEGF and KDR protein and their clinical significance in endometrial carcinoma tissues

目的:分析子宫内膜癌组织COX-2、VEGF和KDR蛋白的表达及临床意义。方法:应用免疫组化法对112例子宫内膜癌组织COX-2、VEGF和KDR蛋白进行检测。结果:112例子宫内膜癌组织COX-2、VEGF和KDR蛋白的阳性率分别为47.32%、66.07%和52.68%,三者表达具有协同性。Ⅱ+Ⅲ+Ⅳ期子宫内膜癌组织COX-2、VEGF和KDR蛋白表达阳性率分别为68%、88%和72%,高于Ⅰ期41.38%、59.77%和47.13%,P<0.05。低分化子宫内膜癌组织COX-2和VEGF蛋白表达阳性率100%,高于中分化61.11%和69.44%以及高分化的29.69%和57.81%,P<0.05。肌层浸润>1/2子宫内膜癌组织COX-2、VEGF和KDR蛋白表达阳性率62.5%、96.88%和84.38%,高于肌层浸润≤1/2者41.25%、53.75%和40%,P<0.05。结论:COX-2、VEGF和KDR蛋白在子宫内膜癌织中的表达水平均较高且具有协同性。COX-2、VEGF及KDR是参与肿瘤发生、发展和转移的重要基因。

OBJECTIVE： To analyze the expressions of COX-2,VEGF and KDR protein in endometrial carcinoma in order to find their clinical significance.METHOD：The immunohistochemical method was used to detect the expressions of COX-2,VEGF and KDR protein.RESULTS： The expression rates were 47.32% for COX-2 detected,66.07% for VEGF detected and 52.68% for KDR detected respectively in 112 patients with endometrial carcinoma.The positive rates of COX-2,VEGF and KDR protein expressions in operative stage Ⅱ＋Ⅲ＋Ⅳ（68%,88% and 72%） were statistically higher than those in stage Ⅰ（41.38%,59.77% and 47.13%,P〈0.05）.The positive rates of COX-2,VEGF protein expression in poorly differentiated group（100% and 100%） were statistically higher than those in moderately and well differentiated groups（61.11% and 29.69%;69.44% and 57.81%,P〈0.05）.The positive rates of COX-2,VEGF and KDR protein expressions in myometrial invasion 〉1/2（62.5%,96.88% and 84.38%） were statistically higher than those in ≤1/2（41.25%,53.75%,40%,P〈0.05）.CONCLUSIONS： The expressions of COX-2,VEGF and KDR protein in endometrial carcinoma are high.COX-2,VEGF and KDR are important genes in the carcinogenesis,development and metastasis of endometrial carcinoma.